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Separation Of Kentucky Bluegrass Cultivars Using Peroxidase Isoenzyme Banding Patterns, David J. Wehner, J. M. Duich, T. L. Watschke

Office of the Dean (CAFES) Scholarship

The peroxidase isoenzyme banding pattern of 15 Kentucky bluegrass (Poa pratensis L.) cultivars was investigated for use in identification. Isoenzymes were separated by polyacrylamide slab gel electrophoresis, stained with benzidine-H₂O₂, and the relative intensities of the bands evaluated with a densitometer. Cultivar separations were made on single seedling samples at the 1% level of confidence using band number, mobility, and intensity ratios. Overall variability of the procedure was evaluated using a horseradish peroxidase standard and the effects of seedling age and seedlot determined. Of the 15 cultivars, 11 could be separated individually, whereas the remaining …