Life Sciences Commons^™

Understanding And Developing Safer Sanitation Agents And Strategies In Food Production Environments, Pragathi Kamarasu

Doctoral Dissertations

Food is a primary resource for survival of human beings, and it is also one of the primary resources for spread of infectious diseases. When both these factors come together it does not only cause ill effects on our health but also causes a burden on the food and agricultural industry. Through the help of science, us researchers and scientists have always tried to reduce this burden. And I hope like a drop of water in the ocean in some way my research contributes towards this. According to WHO currently in 2023 there are about 1 in 10 people falling …

Biological Remediation Of Fragipan, Corey Hale

Honors College Theses

The fragipan layer in the soil is a naturally occurring subsurface layer that restricts water percolation as well as inhibits root growth. A fragipan layer can be found almost ubiquitously across Southwestern Kentucky. This limits how agriculturalists manage soils in this area. It has been recently discovered by the University of Kentucky that Annual Ryegrass roots produce 3,4 dihydroxyphenylpropionic acid (DHPPA) that reacts with this hardpan to weaken the layer. Certain bacteria, such as Escherichia coli, Lactobacilli, and Bifidobacteria can also synthesize DHPPA through a breakdown process of chlorogenic acid. This reaction typically happens in the human digestive tract. …

An Investigation Of The Novel Use Of Bacteriophages To Diagnose And Treat Johne's Disease In Cattle, Max Kevane-Campbell

ORBioM (Open Research BioSciences Meeting)

Johne’s disease is a scourge to dairy farmers all over the world. It is an infectious disease that causes chronic inflammation and lesions along the inside of the small intestine of, primarily, ruminant animals (i.e., cattle and sheep). It is an incurable disease and urgently requires new and radical intervention strategies. Apart from careful on-site farm management practices, little can be offered to farmers to reduce the risk of infection, and nothing short of livestock culling is effective once an animal becomes infected. Currently, there are no vaccines licensed in Ireland or antibiotic treatment strategies available for Johne’s disease. This …

An Investigation Of The Relationship Between Black Soldier Fly Larvae Pathogen Suppression And Growth Substrate In Relation To Maine Agricultural Industries, Haley Morrill

Honors College

Insects are promoted as cost-effective and sustainable protein sources for animal feed. Their utilization may help to avoid a predicted global protein shortage. Black soldier fly (Hermetia illucens) larvae (BSFL) grow on organic wastes, converting these wastes into larval biomass which can fulfill this purpose. Potential benefits of using BSFL to remediate organic wastes include reduction of waste mass and bacterial load, along with the sale of larvae as a protein supplement. BSFL suppress the growth of some Gram-positive and Gram-negative human pathogens in these substrates; though suppression of Bacillus cereus, a spore-forming bacterium that causes food-poisoning, …

The Effects Of Priming Eruca Sativa Seeds With Short-Chain Ahl C6-Hsl At Bard Farm, Shannon M. Ryan

Senior Projects Spring 2021

Many gram-negative bacteria use quorum sensing to assess population densities and cooperate with another. The quorum sensing autoinducers N-acyl homoserine lactones (AHLs) have been found to cause altered gene expression patterns in plants, resulting in increased root and shoot growth as well as induced pathogenic resistance in various species. Researchers have begun exploring the ways AHLs may be used in agricultural systems to reduce the use of environmentally harmful synthetic fertilizers and pesticides. In this study, the effect of priming arugula (Eruca sativa) seeds with the short-chain AHL C6-HSL was investigated at Bard Farm in Annandale-On-Hudson, New York. Leaf lengths …

Integrative Experience: Soil Microbes And The Sustainability Of Organic Agriculture, Kristen Deangelis, Luiz Domeignoz Horta

Microbiology Educational Materials

This curriculum describes a one-unit course designed to fulfill the University of Massachusetts requirement for Integrative Experience as part of the Gen Ed curriculum for undergraduates.

A Research Of Some Pathogen Microorganisms In Traditional Sharri Cheese, Ali Aydin, Endra Luzha

UBT International Conference

Sharri cheese is a traditional hard, fatty and salty type of cheese produced in Sharri, Gora, Opoja and Shtirpca towns in the Sharr Mountains region. Sharri cheese is produced by processing sheep milk, cow milk or their mixture. The aim of this study was to determine the microbiological quality of traditionally and industrially produced Sharri cheese. In total 58 samples of cheese were examined. Staphyloccocus aureus was positive in 57 samples. In a research for Eschericia coli, 34 samples showed result from 1x10¹/mL to 4.5x10⁶/mL. According to our results, Sharri cheese can carry risk for foodborne pathogens and these …

Is Mycoplasma Bovis In Sand Bedding Infectious To Dairy Calves?, David J. Wilson, Thomas J. Baldwin

All Current Publications

Mycoplasmas are unusual bacteria that can infect all ages of cattle, and can cause arthritis, pneumonia, and death. Infected dairy cows may also contract mastitis, metritis, or virtually cease milk production. The most common mycoplasma affecting cattle is M. bovis; there are several other Mycoplasma spp. as well. Because standard microbial culture methods do not isolate Mycoplasma spp., special laboratory methods are needed for diagnosis. Mycoplasma spreads by inhalation and respiratory secretions and also at milking time via contaminated inflations in milking units. Mycoplasma spp. have also been detected in straw, sand, recycled manure, and other bedding, often associated …

Quality Of Fresh And Stored Mares’ Milk, Grażyna Czyżak-Runowska, Jacek Wójtowski, Alicja Niewiadomska, Maria Markiewicz-Keszycka

Articles

Mares’ milk is characterised by unique nutritional profile. In this study, the microbiological analysis of mares’ milk was performed. The presence of total bacteria, total lactic bacteria, Lactobacillus spp., Streptococcus lactis, Salmonella spp. and coliforms was investigated. Moreover, the influence of refrigerated and frozen storage on the total bacteria count, vitamin C, acidity and colour of milk was examined. Pathogenic Salmonella spp. and coliforms were not detected in the raw milk. It was revealed that mares’ milk can be stored for 72 hours under refrigeration at a temperature of +4 °C without reducing its microbiological quality. Most of the physicochemical …

Is Tb Testing Associated With Increased Blood Interferon-Gamma Levels?, Aideen E. Kennedy, Jim O'Mahony, Noel Byrne, John Macsharry, Riona G. Sayers

Department of Biological Sciences Publications

The Republic of Ireland reports a relatively low prevalence of Johne’s disease (JD) compared to international counterparts. Postulated reasons for this include a lower average herd size and a grass-based production system. Ireland also engages in high levels of bovine tuberculosis (bTB) testing. As interferon-gamma (IFN-γ) is believed to play a key role in protecting against JD, it is our hypothesis that administration of purified protein derivative (PPD), as part of the bTB test, is associated with a systemic increase in IFN-γ production, which may potentially limit clinical progression of the disease. We studied 265 cows (202 Friesian and 63 …

Analysis Of Johne’S Disease Elisa Status And Associated Performance Parameters In Irish Dairy Cows, A. E. Kennedy, N. Byrne, A. B. Garcia, Jim O'Mahony, R. G. Sayers

Department of Biological Sciences Publications

Infection with Mycobacterium avium subspecies paratuberculosis (MAP) has been associated with reductions in milk production in dairy cows and sub optimal fertility. The aim of this study was to highlight the production losses associated with testing MAP ELISA positive in Irish dairy cows. Secondary objectives included investigation of risk factors associated with testing MAP ELISA positive. A survey of management practices on study farms was also conducted, with examination of associations between management practices and herd MAP status.

Blood samples were collected from 4188 breeding animals on 22 farms. Samples were ELISA tested using the ID Screen Paratuberculosis Indirect Screening …

Phages Of Non-Diary Lactococci: Isolation And Characterization Of Phi L47, A Phage Infecting The Grass Isolate Lactococcus Lactis Ssp Cremoris Dpc6860, Daniel Cavanagh, Caitríona M. Guinane, Horst Neve, Aidan Coffey, R. Paul Ross, Gerald F. Fitzgerald, Olivia Mcauliffe

Department of Biological Sciences Publications

Lactococci isolated from non-dairy sources have been found to possess enhanced metabolic activity when compared to dairy strains. These capabilities may be harnessed through the use of these strains as starter or adjunct cultures to produce more diverse flavor profiles in cheese and other dairy products. To understand the interactions between these organisms and the phages that infect them, a number of phages were isolated against lactococcal strains of non-dairy origin. One such phage, ΦL47, was isolated from a sewage sample using the grass isolate L. lactis ssp. cremoris DPC6860 as a host. Visualization of phage virions by transmission electron …

Microbial Development In Distillers Wet Grains Produced During Fuel Ethanol Production From Corn (Zea Mays), R. Michael Lehman, Kurt A. Rosentrater

Kurt A. Rosentrater

Distillers grains are coproduced with ethanol and carbon dioxide during the production of fuel ethanol from the dry milling and fermentation of corn grain, yet there is little basic microbiological information on these materials. We undertook a replicated field study of the microbiology of distillers wet grains (DWG) over a 9 day period following their production at an industrial fuel ethanol plant. Freshly produced DWG had a pH of about 4.4, a moisture content of about 53.5% (wet mass basis), and 4 x 10(5) total yeast cells/g dry mass, of which about 0.1% were viable. Total bacterial cells were initially …

Ec92-2308 Principles And Practices For Food Sanitation Programs, Susan S. Sumner, Dianne L. Peters

University of Nebraska-Lincoln Extension: Historical Materials

Food plant sanitation programs will vary depending on the type of product produced. All sanitation programs begin wtih a commitment to construct, upgrade, and maintain the food processing system. Follow federal, state and local regulations. This publication will concentrate on your food plant sanitation program and will cover the following areas: plant and grounds, plant construction, equipment, receiving and storage, processing and packaging, warehousing and shipping, cleaning and sanitizing, personal hygiene and food handling.

Ec92-2307 Food Microbiology/Foodborne Illness, Julie A. Albrecht, Susan S. Sumner

University of Nebraska-Lincoln Extension: Historical Materials

This extension circular discusses the microorganisms in food. Bacteria, yeasts, and mold are microorganisms associated with foods. The individual microorganism cannot be seen without the aid of a microscope. Microorganisms may be classified into three groups according to their activity: beneficial, spoilage, and pathogenic. All three microorganisms will be discussed.