Life Sciences Commons^™

Development Of Mping-Based Activation Tags For Crop Insertional Mutagenesis, Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacob Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh T. Nguyen, Adrian Stec, Lauren A.L. Mcdaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C Nathan Hancock

Department of Agronomy and Horticulture: Faculty Publications

Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis …

Defining Bee Pollinator Community Composition In Tennessee Soybean, Andrew L. Lawson

Masters Theses

Two planting dates of various soybean varieties were planted in Jackson and Knoxville, TN during 2018 and 2019 with the overall intent of surveying the diversity bee (Hymenoptera) genera in these agroecosystems and also to assess the potential for using late maturing soybean as a food resource for bees during the dearth of floral resources that often occurs during the fall. We also investigated how manipulating planting dates and soybean variety selection affected the occurrence of insect pests that occurred in the soybean.

Both active (netting) and passive (bee bowls and blue-vane traps) sampling were used to collect the bees, …

Quantitative Trait Loci Analysis Of A Ril Soybean Population To Determine Chromosomal Regions Governing Seed Protein, Oil, And Linolenic Acid Content, Ronald E. Moore

Masters Theses

180 RILs (recombinant inbred lines) segregating for protein, oil, and fatty acids were produced from a cross between TN12-4098 and TN13-4303. These lines were grown across three locations spread horizontally across Tennessee: Research Education Center at Milan (RECM), Highland Rim Research and Education Center (HRREC), and East Tennessee Research and Education Center (ETREC) in 2018 and 2019. 21 quantitative trait loci (QTLs) spanning 7 chromosomes were found using WinQTLCart2.5.

Quality Differences And Correlation Analysis Of Natto Fermented From Soybean Seeds With Different Protein Content, Lin Yong-Shan, Gao Xiang-Yang, Cheng Jia, Zeng Jian, Chen Rui-Zhi, Liu Yu-Long, Yang Cun-Yi

Food and Machinery

Seven genotype soybean seeds with different protein content were using as the raw material for natto fermentation. The study was focus on the difference of the sensory quality and properties of mucilage on the surface of natto after fermented by Bacillus subtilis natto. Then the relationship between the quality of mucilage and the quality traits of soybean seeds was analyzed. The results showed that, the sensory score and stretch length of natto mucilage fermented from five different genotype soybean except green soybean YSG-10 and black soybean YSB-37 were significantly negatively correlated with seeds protein content respectively (r are 0.953, 0.946 …

Cover Cropping In Soybean-Corn Rotation System: Economic, Agronomic, And Soil Fertility Impact, Jose Rodolfo Mite

LSU Doctoral Dissertations

Planting winter-annual cover crops prevent soil erosion, reduces water runoff, and improves soil structure and soil quality. This research was conducted from 2017 to 2019 to evaluate the nutrient turnover of different species of cover crops in soils under different row crop production systems in Northeast and Central Louisiana. In Northeast Louisiana (Site 1, 2, and 3), treatments (cover crops and no cover crop) were arranged in a strip trial with three replications. At the Ben Hur Research Station, the treatments included three planting dates (September, October, and November) with [7 kg ha^-1 of phosphorus (P) and potassium (K)] …

Assessing Approaches For Stratifying Producer Fields Based On Biophysical Attributes For Regional Yield-Gap Analysis, Spyridon Mourtzinis, Patricio Grassini, Juan I. Rattalino Edreira, José F. Andrade, Peter M. Kyveryga, Shawn P. Conley

Department of Agronomy and Horticulture: Faculty Publications

Large databases containing producer field-level yield and management records can be used to identify causes of yield gaps. A relevant question is how to account for the diverse biophysical background (i.e., climate and soil) across fields and years, which can confound the effect of a given management practice on yield. Here we evaluated two approaches to group producer fields based on biophysical attributes: (i) a technology extrapolation domain spatial framework (‘TEDs’) that delineates regions with similar (long-term average) annual weather and soil water storage capacity and (ii) clusters based on field-specific soil properties and weather during each crop …

Investigation Of The Individual Soybean Root Nodule Microbiome, Parris Mica Mayhood

MSU Graduate Theses

Soybean is a valuable crop that establishes a symbiotic association with nitrogen-fixing microorganisms. Previous research suggests members of the genus Bradyrhizobium are the major nitrogen-fixing inhabitants of soybean root nodules. However, results from recent studies identified the presence of other bacteria, so called non-rhizobial endophytes (NREs), inside root nodules. All previous research has pooled nodules of different plants for bacterial community analysis, but this study investigated the presence and relative abundance of rhizobia and NREs within multiple root nodules of a single plant. Three sites were selected on Kendrick Farm in Springfield, MO, where three plants and rhizosphere soil were …

Cloning And Expression Analysis Of Gmwrky86 In Soybean, Wang Jing, Fu Jing, Li Xiao-Wei

Food and Machinery

In order to study the biological function of GmWRKY86 gene in soybean on development and resistance against abiotic stress, PCR products of its opening reading frame, ORF) were used to clone GmWRKY86 gene, and the gene expressions in different tissues were detected by using real-time fluorescence quantitative PCR (qRT-PCR). Sequence analysis showed that the GmWRKY86 gene can encode 523 amino acids with an isoelectric point of 8.15, a molecular weight predicted to be 56.82 kDa, and an open reading frame of 1 572 bp. GmWRKY86 protein has two highly conserved domains, and the GmWRKY86 gene contains three introns and four …

Soybean Response To Water: Trait Identification And Prediction, Shawn Jenkins

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The rising demand for soybean [Glycine Max (L.) Merrill] taken in consideration with current climatic trends accentuates the importance of improving soybean seed yield response per unit water (WP). To further our understanding of the quantitative WP trait, a multi-omic approach was implemented for improved trait identification and predictive modeling opportunities. Through the evaluation of two recombinant inbred line populations jointly totaling 439 lines subjected to contrasting irrigation treatments, informative agronomic, phenomic, and genomic associations were identified. Across both populations, relationships were identified between lodging at maturity (r = -0.58, H = 0.86), canopy to air temperature differential …

Organic Soybean Variety Trial, Heather Darby, Sara Ziegler, Ivy Krezinski, Rory Malone

Northwest Crops & Soils Program

In 2020, the University of Vermont Extension Northwest Crops and Soils Program evaluated yield and quality of short season organic soybean varieties at Borderview Research Farm in Alburgh, VT. Soybeans can be grown for human consumption, animal feed, and biodiesel. As farmers look to reduce feed costs or diversify markets, soybean acreage across Vermont is increasing. Local research is needed to identify varieties that are best adapted to this region. In an effort to support and expand the local soybean market throughout the northeast, the University of Vermont Extension Northwest Crop and Soils (NWCS) Program established a trial in 2020 …

Soybean Cover Crop Trial, Heather Darby, Ivy Krezinski, Sara Ziegler

Northwest Crops & Soils Program

In 2020, the University of Vermont Extension Northwest Crops and Soils Program investigated the impact of various cover crop mixtures on the subsequent soybean crop’s yield and quality at Borderview Research Farm in Alburgh, VT. Soybeans are grown for human consumption, animal feed, and biodiesel and can be a useful rotational crop in corn silage and grass production systems. As cover cropping expands throughout Vermont, it is important to understand the potential benefits, consequences, and risks associated with growing cover crops in various cropping systems. In an effort to support the local soybean market and to gain a better understanding …

Conventional Soybean Performance Trials Summary, Heather Darby

Northwest Crops & Soils Program

Maturity range (0 – 2.1) of soybean varieties in Alburgh, VT

Planting Date: 05-21-2020 Harvest Date: 10-15-2020

Impact Of Planting Date And Variety On Soybean Yield, Heather Darby, Sara Ziegler, Ivy Krezinski, Rory Malone

Northwest Crops & Soils Program

In 2020, the University of Vermont Extension Northwest Crops and Soils Program investigated the impact of planting date and variety on soybean yield and quality at Borderview Research Farm in Alburgh, VT. Soybeans can be grown for human consumption, animal feed, and biodiesel. Livestock farmers are interested in producing more of their own grains and as a result, soybean acreage in Vermont is increasing. Given the short growing season in Vermont, it is important to understand optimum planting dates to obtain the highest yields. In an effort to support and expand the local soybean market throughout the northeast, the University …

Organic Soybean Performance Trials Summary, Heathe Darby

Northwest Crops & Soils Program

Maturity range (0.6 – 1.8) of soybean varieties in Alburgh, VT

Planting Date: 05-21-2020 and Harvest Date: 10-14-2020

Influence Of Burner Position On Temperature Distribution In Soybean Flaming, Miloš Miloš Rajkovic, Goran Malidža, Strahinja Stepanovic, Marko Kostic, Kristina Petrovic

West Central Research and Extension Center, North Platte

The main objective of this study was to identify optimal burner orientation for a newly designed flame cultivator by quantifying the flame temperature distributions of cross, back, and parallel position of burners at different heights of the soybean canopy (distance from the soil surface). Flame temperatures were measured within-row for three burner orientations at seven propane doses (20–100 kg/ha) and eight different canopy heights (0–18 cm above soil surface). Soybean plants in V3 growth stage were flamed with the same doses and burner orientations, and 28 days after treatment (DAT) crop injury (0%–100%), plant height (cm), dry matter (g) and …

In-Season Crop Management Effect On Soybean Yield And Grain Quality In Eastern South Dakota, Kelsey Bergman

Electronic Theses and Dissertations

Soybean [Glycine max (L.) Merr.] yield has increased over time by introduction of newer varieties and improved agronomic practices. However, grain protein concentration has been decreasing at the same time. New field studies in 2018 and 2019 investigated inseason crop management practices effect such as use of foliar protection application, fertilizer application, different maturity length or different planting dates on grain yield and grain quality. The studies were established near Brookings, SD and Beresford, SD. In-season treatments were targeting nutrient availability or protecting soybean canopy during the grain filling period. Such treatments included the use of fungicide, insecticide, or supplying …

Burning With Potential: Understanding The Relationship Between Biochar And Agriculture Of The Northern Glaciated Plains Ecoregion, Kaitlyn Abrahamson

Electronic Theses and Dissertations

Achieving global sustainable agriculture is one of the most incredible challenges of this century, yet many continue to try to solve this problem through the development of precision technologies. Biotechnologies, such as biochar, can perform like a precision technology while protecting agricultural land from soil erosion and fertility loss. The Northern Glaciated Plains ecoregion of the United States is little researched in the benefits from the use of biochar through improved soil nutrient capture and water retention, crop health improvements, and yield increases. The study plot has four sections of corn stover biochar and eight sections of control sections. This …

Insufficient Nitrogen Supply From Symbiotic Fixation Reduces Seasonal Crop Growth And Nitrogen Mobilization To Seed In Highly Productive Soybean Crops, Nicolas Cafaro La Menza, Juan Pablo Monzon, John L. Lindquist, Timothy J. Arkebauer, Johannes M.H. Knops, Murray Unkovich, James E. Specht, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen (N) supply can limit the yields of soybean [Glycine max (L.) Merr.] in highly productive environments. To explore the physiological mechanisms underlying this limitation, seasonal changes in N dynamics, aboveground dry matter (ADM) accumula- tion, leaf area index (LAI) and fraction of absorbed radiation (fAPAR) were compared in crops relying only on biological N2 fixation and available soil N (zero-N treatment) versus crops receiving N fertilizer (full-N treatment). Experiments were conducted in seven high-yield environments without water limitation, where crops received optimal management. In the zero-N treatment, biological N2 fixation was not sufficient to meet the N demand of …

Management Of Stink Bugs (Hemiptera: Pentatomidae) On Soybean In Kentucky, Yaziri Gonzalez

Theses and Dissertations--Entomology

Phytophagous stink bugs (Hemiptera: Pentatomidae) are pests of significant importance to soybean, Glycine max (L.), because they can cause severe losses in seed quality, ultimately affecting yield. Damage to soybean is caused through a process called extra-oral digestion. This occurs when the adults and nymphs of stink bugs pierce plant tissues with their mandibular and maxillary stylets, insert digestive enzymes, break down tissue, and extract the digested plant fluid. Three endemic stink bug species of North America are present in Kentucky: green stink bug (Chinavia hilaris), brown stink bugs (Euschistus species complex), and red-shouldered stink bug (Thyanta custator) …