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Detection Of Four Apple Viruses By Elisa And Rt-Pcr Assays In Turkey, Kadri̇ye Çağlayan, Çi̇ğdem Ulubaş Serçe, Mona Gazel, Wilhelm Jelkmann
Detection Of Four Apple Viruses By Elisa And Rt-Pcr Assays In Turkey, Kadri̇ye Çağlayan, Çi̇ğdem Ulubaş Serçe, Mona Gazel, Wilhelm Jelkmann
Turkish Journal of Agriculture and Forestry
Plant samples were collected from the main apple growing provinces of Turkey in order to evaluate the incidence of 4 important apple virus diseases during spring 2004. Collected leaves and shoots were tested using enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) for Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV) and Apple chlorotic leaf spot virus (ACLSV). Since no commercial antiserum is available, Apple stem pitting virus (ASPV) was tested only using RT-PCR and the results were compared. A total of 174 apple samples were collected from varietal collections belonging to governmental and university …