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Pesticide Analysis In Vegetables Using Quechers Extraction And Colorimetric Detection, D. S. G. Neufeld, N. Åkerson, D. Barahona
Pesticide Analysis In Vegetables Using Quechers Extraction And Colorimetric Detection, D. S. G. Neufeld, N. Åkerson, D. Barahona
Virginia Journal of Science
A novel combination of extraction and detection methods is demonstrated for pesticide residue analysis in vegetable samples. Acetylcholinesterase (AChE) inhibition was used as a simple colorimetric test for organophosphates/carbamates (OP/C), and was tested with extracts from the widely-used QuEChERS extraction method. In the absence of pesticide, diluted (50% with water) acetonitrile did not inhibit enzyme activity, demonstrating the compatibility of this extraction solvent with the AChE inhibition test. QuEChERS extraction of chlorpyrifos-spiked tomato, spinach and lettuce samples indicated a high sensitivity to OP/C, with AChE inhibition occurring in the ppb range. The applicability of this method combination was tested by …