Life Sciences Commons^™

Genetic Characterization Of North American Populations Of The Wheat Curl Mite And Dry Bulb Mite, Gary L. Hein, Roy French, Benjawan Siriwetwiwat, James W. Amrine

Department of Entomology: Faculty Publications

The wheat curl mite, Aceria tosichella Keifer, transmits at least three harmful viruses, wheat streak mosaic virus (WSMV), high plains virus (HPV), and Triticum mosaic virus (TriMV) to wheat (Triticum aestivum L.) throughout the Great Plains. This virus complex is considered to be the most serious disease of winter wheat in the western Great Plains. One component of managing this disease has been developing mite resistance in wheat; however, identification of mite biotypes has complicated deployment and stability of resistance. This biotypic variability in mites and differential virus transmission by different mite populations underscores the need to better understand …

Seasonal Population Dynamics Of The Potato Psyllid (Hemiptera: Triozidae) And Its Associated Pathogen “Candidatus Liberibacter Solanacearum” In Potatoes In The Southern Great Plains Of North America, John A. Goolsby, John J. Adamczyk Jr., J. M. Crosslin, Noel N. Troxclair, J. R. Ancisco, Gerhard G. Bester, J. D. Bradshaw, Edsel D. Bynum Jr., L. A. Carpio, Don C. Henne, Ankush Joshi, Joseph E. Munyaneza, Pat Porter, Phillip E. Sloderbeck, J. R. Supak, C. M. Rush, F. J. Willett, B. J. Zechmann, B. A. Zens

Department of Entomology: Faculty Publications

The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), and its associated pathogen “Candidatus Liberibacter solanacearum” (Ca. L. solanacearum), the putative causal agent of zebra chip (ZC) disease in potatoes (Solanum tuberosum L.), were sampled in commercial potato fields and untreated control plots for 3 yr in multiple locations in Texas, Kansas, Nebraska, and Colorado. Populations of the potato psyllid varied across years and across potato growing regions. However, the percentage of potato psyllids infected with Ca. L. solanacearum although variable across years, was consistently highest in the Lower Rio Grande Valley of Texas (LRGV), the reported overwintering …

Insecticidal Control Of Bemisia Tabaci (Hemiptera: Aleyrodidae) Transmitting Carlavirus On Soybeans And Detection Of The Virus In Alternate Hosts, Difabachew K. Belay, Randy M. Huckaba, Axel M. Ramirez, Jose C. V. Rodrigues, John E. Foster

Department of Entomology: Faculty Publications

A Carlavirus transmitted by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is an important disease of soybean nurseries in Puerto Rico causing substantial germplasm losses. Insecticide bioassay experiments were conducted at Dow AgroSciences (DAS) Research Station, Santa Isabel, Puerto Rico, either by spraying insecticides on B. tabaci infested soybean leaves or introducing B. tabaci adults onto insecticide-sprayed soybean leaves. Moreover, host plants were surveyed to detect the virus in overwintering hosts that serve as a source of inoculums. The direct spray experiment showed that Nuprid 2F (Imidacloprid), Capture 2 EC (Bifenthrin), Thionex (Endosulfan), Lannate LV (Methomyl), and Dimethoate gave good level ( …

Genetic Differentiation Of Western Corn Rootworm Populations (Coleoptera: Chrysomelidae) Relative To Insecticide Resistance, Hong Chen, Haichuan Wang, Blair D. Siegfried

Department of Entomology: Faculty Publications

Abstract

As the single most important pest of field corn, Zea mays L., throughout most of the Corn Belt, the western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), has undergone repeated selection for resistance to a variety of insecticides that persist widely among Nebraska populations. In this study, we used 11 microsatellite markers to genotype two populations with high levels of resistance to methyl-parathion and aldrin (Polk and Stromsburg), two populations with low and intermediate levels of resistance (Mead and Clay Center) from Nebraska, and one population from outside the Corn Belt (Safford, Arizona). The genetic diversity measured by …

Mortality Impact Of Mon863 Transgenic Maize Roots On Western Corn Rootworm Larvae In The Field, T. L. Clark, D. L. Frank, B. W. French, Lance J. Meinke, D. Moellenbeck, T. T. Vaughn, B. E. Hibbard

Department of Entomology: Faculty Publications

Mortality of western corn rootworm (Diabrotica virgifera virgifera LeConte) due to feeding on MON863 transgenic maize (Zea mays L.) expressing the Cry3Bb1 protein was evaluated at three Missouri sites in both 2003 and 2004 and at one site each in South Dakota, Nebraska, and Iowa in 2004. To do this, survivorship relative to survivorship on isoline maize (i.e., the same genetic background, but without Cry3Bb1) was evaluated. Comparisons were made using low (1650–2500 eggs/m) and high (3300–3500 eggs/m) western corn rootworm egg densities. Significantly fewer beetles were recovered from MON863 than from isoline maize. Emergence from MON863 as …

Using Video-Tracking To Assess Sublethal Effects Of Pesticides On Honey Bees (Apis Mellifera L.), Bethany S. Teeters, Reed M. Johnson, Marion D. Ellis, Blair D. Siegfried

Department of Entomology: Faculty Publications

Concern about the role of pesticides in honey bee decline has highlighted the need to examine the effects of sublethal exposure on bee behaviors. The video-tracking system EthoVisionXT (Noldus Information Technologies) was used to measure the effects of sublethal exposure to tau-fluvalinate and imidacloprid on honey bee locomotion, interactions, and time spent near a food source over a 24-h observation period. Bees were either treated topically with 0.3, 1.5, and 3 μg tau-fluvalinate or exposed to 0.05, 0.5, 5.0, 50, and 500 ppb imidacloprid in a sugar agar cube. Tau-fluvalinate caused a significant reduction in distance moved at all dose …

Larval Survival And Plant Injury Of Cry1ab-Susceptible, -Resistant, And -Heterozygous Genotypes Of The Sugarcane Borer On Transgenic Corn Containing Single Or Pyramided Bt Genes, David S. Wangila, B. Rogers Leonard, Yaoyu Bai, Graham P. Head, Fangneng Huang

Department of Entomology: Faculty Publications

Transgenic corn (Zea mays L.) products expressing multiple Bt proteins targeting a same group of insect pests have become commercially available in the U.S. The first commercialized pyramided Bt corn technologies for managing lepidopteran pests include Genuity® VT Triple Pro™ and Genuity® SmartStax™. In this study, larval survival and plant injury of Cry1Ab-susceptible (Cry1Ab-SS), -resistant (Cry1Ab-RR), and -heterozygous (Cry1Ab-RS) genotypes of the sugarcane borer, Diatraea saccharalis F., on five commercial corn hybrids were evaluated in a leaf tissue bioassay and two greenhouse trials during 2010–2011. The five hybrids included two non-Bt corn and three Bt corn hybrids representing three …

Susceptibility Of Cry1ab Maize-Resistant And -Susceptible Strains Of Sugarcane Borer (Lepidoptera: Crambidae) To Four Individual Cry Proteins, Liping Zhang, Fangneng Huang, B. Rogers Leonard, Mao Chen, Thomas Clark, Yu Cheng Zhu, David S. Wangila, Fei Yang, Ying Niu

Department of Entomology: Faculty Publications

Sugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F2 families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac, and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) …

Enabling Learning And Enhancing Education In Ipm In The Midst Of Dwindling Financial Resources: Why Open Access To Resources And Publishing Could Be A Strategic Step To Support Students Today And Tomorrow?, Ousmane Youm

Department of Entomology: Faculty Publications

When I was invited to join the editorial board of the journal Entomology, Ornithology & Herpetology (EOH), I read carefully the note coming with the invitation and also looked at the content and scope. Then two things came into my mind as I stopped a few minutes to reflect on a couple of issues: (1) how the new journal links and is relevant to Integrated Pest Management—IPM (for those who understand its definition)—and (2) the issue of open access and relevance in the current situation of an ailing global economy, particularly in the vulnerable developing nations.

In the latter, more …

Pheromone–Mediated Mating Disruption In The Millet Stem Borer, Coniesta Ignefusalis (Lepidoptera: Pyralidae), Ousmane Youm, Yacouba Maliki, David R. Hall, Dudley I. Farman, John E. Foster

Department of Entomology: Faculty Publications

The millet stem borer, Coniesta ignefusalis Hampson (Lepidoptera: Pyralidae), is a major pest of pearl millet in the Sahelian region of Africa. The female sex pheromone has been identified and synthesized, and previous research had shown that the synthetic pheromone could cause high levels of reproductive communication disruption in small plots when released at rates of 640 mg/ha/day, using PVC resin formulation renewed every seven days to maintain efficiency. In the present research, in experiments in farmers’ fields in Niger, 86.8% (SE = 2.6%) communication disruption was achieved when polyethylene vials loaded with 0.5 mg pheromone at 400 dispensers/ha were …