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A Crna Probe Detects Prunus Necrotic Ringspot Virus In Three Peach Cultivars After Micrografting And In Peach Shoots Following Long-Term Culture At 4°C, K. Heuss, Q. Liu, F.A. Hammerschlag, R.W. Hammond
A Crna Probe Detects Prunus Necrotic Ringspot Virus In Three Peach Cultivars After Micrografting And In Peach Shoots Following Long-Term Culture At 4°C, K. Heuss, Q. Liu, F.A. Hammerschlag, R.W. Hammond
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
As part of a program to develop transgenic peach (Prunus persica L. Batsch) cultivars with resistance to Prunus necrotic ringspot virus (PNRSV), we are testing a system for measuring virus in peach shoot cultures. Micrografting in vitro is used for inoculation and slot-blot hybridization, with a digoxigenin (DIG)-labeled cRNA probe complementary to the 5´ open reading frame (ORF) of PNRSV RNA 3, for detection. In this study, we investigated whether infected shoots maintain virus infection over long periods of culture at 4°C and if PNRSV-infected ‘Suncrest’ shoot cultures can serve as graft bases to transmit virus equally well into …