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Nanofabrication And Characterization Of An Enzyme-Less Electrochemical Biosensor For Creatinine Detection, Anas Belharsa
Nanofabrication And Characterization Of An Enzyme-Less Electrochemical Biosensor For Creatinine Detection, Anas Belharsa
Electronic Theses and Dissertations, 2020-
This study will reveal the fabrication and development of an enzyme-less biosensor for creatinine detection. The biosensor involves a periodically patterned nano-porous TiO2 deposited with Au nanoparticles via e-beam evaporation and a layer of Imprinted Polymer (IP) of acrylamide and bis-acrylamide to obtain a heterostructure of I-Au-TiO2. The detection methods of creatinine are based on electrochemical measurements using Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV). The IP-Au-TiO2 sensor shows a detection LOD of 0.0949ng/mL and 0. 0.218ng/mL for EIS and DPV measurements, respectively. The nanofabricated biosensor was tested in the presents of urea, glucose, lactose, …
Synthesis Of Ruthenium Bipyridine Conjugated Antibiotic For Fluorescence Lifetime Imaging And Spectroscopy Aided Tracking In Citrus, Ryan Parente
Electronic Theses and Dissertations, 2020-
Antibiotic treatment of systemic bacterial plant pathogens is an established practice in many crops; however, in citrus it has only recently become available for growers to utilize against Huanglongbing disease. The preliminary efficacy of these treatments is uncertain due to the inability to track their presence in phloem. The need to monitor their movement in planta, especially their presence and translocation in vascular tissue, is a necessary step in clarifying their effectiveness. Previous work has shown the value of Fluorescence Lifetime Imaging (FLIM) in distinguishing between fluorescent probes and plant tissue autofluorescence, which is normally a barrier in photochemical studies …
The Synthesis Of Palladium-Platinum Core-Shell Nanoparticles With High Catalytic Peroxidase Activities For Biosensing Applications, Edwin Davidson
The Synthesis Of Palladium-Platinum Core-Shell Nanoparticles With High Catalytic Peroxidase Activities For Biosensing Applications, Edwin Davidson
Electronic Theses and Dissertations, 2020-
In recent years, peroxidase mimic nanostructures have attracted special interest due to their low cost, high stability, and high catalytic activity. Herein, we demonstrate the use of Pd-Pt nanocubes (NCs) to achieve the rapid and sensitive colorimetric detection of ascorbic acid (AA), commonly known as vitamin C. The colored signal generated by the catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by the decomposition of hydrogen peroxide (H2O2) is quenched in the presence of AA based on its antioxidant property. This colorimetric method attains a detection limit of 0.40 µM and a linear range from 0 to 15 µM, with good linearity. To …