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Full-Text Articles in Engineering
Beneficial Mutations For Carotenoid Production Identified From Laboratory-Evolved Saccharomyces Cerevisiae, Avinash Godara, Maria Alejandra Gomez Rodriguez, Joshua Weatherston, George Peabody, Hung-Jen Wu, Katy Kao
Beneficial Mutations For Carotenoid Production Identified From Laboratory-Evolved Saccharomyces Cerevisiae, Avinash Godara, Maria Alejandra Gomez Rodriguez, Joshua Weatherston, George Peabody, Hung-Jen Wu, Katy Kao
Faculty Publications
Adaptive laboratory evolution (ALE) is a powerful tool used to increase strain fitness in the presence of environmental stressors. If production and strain fitness can be coupled, ALE can be used to increase product formation. In earlier work, carotenoids hyperproducing mutants were obtained using an ALE strategy. Here, de novo mutations were identified in hyperproducers, and reconstructed mutants were explored to determine the exact impact of each mutation on production and tolerance. A single mutation in YMRCTy1-3 conferred increased carotenoid production, and when combined with other beneficial mutations led to further increased β-carotene production. Findings also suggest that the ALE …
Cgste11 Mediates Cross Tolerance To Multiple Environmental Stressors In Candida Glabrata, Mian Huang, Jibran Khan, Manpreet Kaur, Julian Daniel Torres Vanega, Orlando Andres Aguilar Patiño, Anand Ramasubramanian, Katy Kao
Cgste11 Mediates Cross Tolerance To Multiple Environmental Stressors In Candida Glabrata, Mian Huang, Jibran Khan, Manpreet Kaur, Julian Daniel Torres Vanega, Orlando Andres Aguilar Patiño, Anand Ramasubramanian, Katy Kao
Faculty Publications
Candida glabrata is a human commensal and an opportunistic human fungal pathogen. It is more closely related to the model yeast Saccharomyces cerevisiae than other Candida spp. Compared with S. cerevisiae, C. glabrata exhibits higher innate tolerance to various environmental stressors, including hyperthermal stress. Here we investigate the molecular mechanisms of C. glabrata adaptation to heat stress via adaptive laboratory evolution. We show that all parallel evolved populations readily adapt to hyperthermal challenge (from 47 °C to 50 °C) and exhibit convergence in evolved phenotypes with extensive cross-tolerance to various other environmental stressors such as oxidants, acids, and alcohols. Genome …
Effects Of Seawater On Carotenoid Production And Lipid Content Of Engineered Saccharomyces Cerevisiae, Yuqi Guo, Shangxian Xie, Joshua Yuan, Katy Kao
Effects Of Seawater On Carotenoid Production And Lipid Content Of Engineered Saccharomyces Cerevisiae, Yuqi Guo, Shangxian Xie, Joshua Yuan, Katy Kao
Faculty Publications
The use of seawater in fermentation can potentially reduce the freshwater burden in the bio-based production of chemicals and fuels. We previously developed a Saccharomyces cerevisiae carotenoids hyperproducer SM14 capable of accumulating 18 mg g−1 DCW (DCW: dry cell weight) of β-carotene in rich media (YPD). In this work, the impacts of seawater on the carotenoid production of SM14 were investigated. When using nutrient-reduced media (0.1× YNB) in freshwater the β-carotene production of SM14 was 6.51 ± 0.37 mg g−1 DCW; however in synthetic seawater, the production was increased to 8.67 ± 0.62 mg g−1 DCW. We found that this …