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Nmr Metabolomics For Optimizing Cell-Free Protein Synthesis, Angela M. Campo
Nmr Metabolomics For Optimizing Cell-Free Protein Synthesis, Angela M. Campo
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Cell-Free Protein Synthesis (CFPS) has been utilized by biochemists to produce a variety of chemicals and therapeutics. While CFPS has spawned research in the biochemistry and medical communities, there are still unknown issues with interlaboratory variability with the technique. This work explored the black box nature of CFPS reactions by analyzing the CFPS reactions in situ with Nuclear Magnetic Resonance (NMR) spectroscopy. Aim 1 developed the protocol for conducting NMR experiments on E. coli cell-free reactions as well as a data analysis pipeline. This was accomplished with 1H NMR, capturing metabolite changes over time. The 1D NOESY experiment proved to …
Ab Initio Modeling Of An Electron Transport Layer Interface In Hybrid Perovskite Solar Cells, Krantikumar Subhash Pawar
Ab Initio Modeling Of An Electron Transport Layer Interface In Hybrid Perovskite Solar Cells, Krantikumar Subhash Pawar
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Hybrid organic-inorganic perovskite solar cell is an emerging technology which has shown the fastest advancement in power conversion efficiency within a few years since introduction, thus making it one of the clean energy breakthroughs. These cells are based on thin-film technology which makes them suitable to manufacture using low-cost solution processing methods. As these types of cells are easily tunable with the selection of different materials, interfacial engineering is an important approach to increasing their efficiency. One of the main hurdles in this regard is the loss caused by the recombination of separated charges. An approach to tackle these issues …