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Optimization Of Sequential Purification Of Beta-Glucosidase From Tricoderma Reesei In Aqueous Two-Phase System, Shalini Gautam
Optimization Of Sequential Purification Of Beta-Glucosidase From Tricoderma Reesei In Aqueous Two-Phase System, Shalini Gautam
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A novel sequential technique was developed for the purification of a valuable enzyme, beta-glucosidase, from microorganism Tricoderma reesei. The fungus T. reesei produces cellulose degrading enzymes, called cellulases: beta-glucosidase, endo-glucanase and exo-glucanase and low molecular weight proteins. For specific applications, the enzyme must be separated from other contaminants. The sequential technique, that included affinity precipitation with chitosan followed by separation with an aqueous two-phase system (ATPS), was implemented for the purification of beta-glucosidase from the culture filtrate of T. reesei.
The cultivation medium (nutrient) was optimized for the production of betaglucosidase from T. reesei cell culture. Treatment of the …