Engineering Commons^™

Lateral Migration Of A Solid Particle In Shear Flow, Wutthapong Jiraporncharoen

Masters Theses

Lateral migration of solid particles in shear flow is observed in various industrial applications. Suspensions in a blade coater, a device used to apply coating pigment to a base paper to improve paper properties, is an example. Particle hydrodynamics will affect the quality of coated paper and runnability of the coater especially when the speed of the coating machine is very high or the pigment concentration is increased.

Many factors will affect the particle migration. The wall repulsion or lubrication effect forces the particle away from a wall to the channel center. The inertial effects are increased by increasing the …

Pilot Mental Workload Calibration, Jeremy B. Noel

Theses and Dissertations

Predicting high pilot mental workload is important to the U.S. Air Force because lives and aircraft can be lost when errors are made during periods of mental overload and task saturation. Current research efforts use psychophysiological measures such as electroencephalography, cardiac, ocular, and respiration measures in an attempt to identify and predict mental workload levels. The primary focus of this effort is the development of a calibration scheme that allows a small subset of salient psychophysiological features developed using actual flight data for one pilot on a given day to accurately classify pilot mental workload for a separate pilot on …

Characterization Of The Biological Activities Of Recombinant Fusion Protein Green Fluorescent Protein/Human Zona Pellucida Protein 3 (Gfp/Hzp3), Zhiyong Lin

Theses and Dissertations in Biomedical Sciences

Despite numerous reports indicating the successful production of bioactive recombinant ZP3, no report has shown the rhZP3 having direct binding activity with human sperm. Recombinant ZP3 generated from our previous study displayed binding activity with human sperm through indirect evidence from hemizona assay (HZA).

This present study focused on the production of recombinant ZP3 with direct binding activity with human sperm. Through the application of a pEGFP expression vector, fusion protein GFP/ZP3 was successfully generated and expressed. The expression of GFP/ZP3 was evidenced by RT-PCR and western blot. The fusion protein was partially purified by Ni-NTA affinity column from cell …