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Design Of A Micro-Focus X-Ray Source Validated By Model Protein Crystals, Lavanya Ramachandran
Design Of A Micro-Focus X-Ray Source Validated By Model Protein Crystals, Lavanya Ramachandran
Theses and Dissertations
The main objective of this thesis was to build a Laboratory Microfocus X-ray Source for Protein Crystallography (LMXS-PC), an X-ray source that would provide prospective users programmatic control and remote capability.
A study of the crystal was conducted using Photoactive Yellow protein (PYP) and cytochrome c nitrite reductase (ccNiR). The instrument was then tested by mounting the crystal in the instrument and collecting data.
The other objective of my research work was to determine the crystal structure of PYP using X-ray crystallography techniques. I attempted many trials in PYP crystal in an effort to achieve a high-quality diffraction pattern; however, …