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Development Of A 3d-Printed Microfluidic Droplet-On-Demand System For The Deterministic Encapsulation And Processing Of Biological Materials, Chandler A. Warr
Development Of A 3d-Printed Microfluidic Droplet-On-Demand System For The Deterministic Encapsulation And Processing Of Biological Materials, Chandler A. Warr
Theses and Dissertations
The growing threat of antimicrobial resistance is among the largest concerns in the world today. One method under development to combat this issue is the encapsulation of microbes in microfluidic droplets for single-cell testing. This method may be able to circumvent the need for a traditional positive cell culture which consumes the majority of the testing time using current diagnostic methods. This dissertation presents a method by which to deterministically encapsulate microbes using an artificial intelligence object detection algorithm and a Droplet-On-Demand microfluidic device. To accomplish this, the Droplet-On-Demand microfluidic device was first developed using a unique 3D-printing manufacturing method. …
Developing Ultra-High Resolution 3d Printing For Microfluidics, Kent Richard Hooper
Developing Ultra-High Resolution 3d Printing For Microfluidics, Kent Richard Hooper
Theses and Dissertations
Building upon previous research on Digital Light Projection (DLP) 3D printing for microfluidics, in this thesis I performed the detailed design and fabrication of a novel DLP 3D printer to increase resolution and device footprint flexibility. This new printer has a pixel resolution twice that of our group’s previous printers (3.8 μm vs 7.6 μm). I demonstrated a new state of the art for minimum channel width, reducing the minimum width to 15 μm wide (and 30 μm tall). This is an improvement over the previous smallest width of 20 μm. This printer also has the capacity to perform multiple …
Optofluidic Manipulation With Nanomembrane Platforms Used For Solid-State Nanopore Integration, Zachary J. Walker
Optofluidic Manipulation With Nanomembrane Platforms Used For Solid-State Nanopore Integration, Zachary J. Walker
Theses and Dissertations
Nanopore technology has introduced new techniques for single particle detection and analysis. A nanopore consists of a small opening in a membrane on the nanometer scale. Nanopores are found in nature and are utilized for transporting molecules through biological membranes. Researchers have been able to mimic naturally forming biological nanopores and utilize them for a variety of sensing applications. Nanopores, fabricated either organically or inorganically, can be used for detecting biomarkers such as proteins, nucleic acids, and metabolites that translocate the membrane by way of the nanopore. Constant ionic current flow is measured through the nanopore by way of a …
A New Approach For 3d Printed Microfluidic Device Design Based On Pre-Defined Components, Cassandra Ester Slaugh
A New Approach For 3d Printed Microfluidic Device Design Based On Pre-Defined Components, Cassandra Ester Slaugh
Theses and Dissertations
3D printing for microfluidic device fabrication has received considerable interest in recent years, in part driven by the potential to dramatically speed up device development by reducing device fabrication time to the minutes timescale. Moreover, in contrast to traditional cleanroom-based fabrication processes that require manual production and stacking of a limited number of layers, 3D printing allows full use of the 3D fabrication volume to lay out microfluidic elements with complex yet compact 3D geometries. The Nordin group has successfully developed multiple generations of high resolution printers and materials for microfluidic devices that achieve this vision. However, because of the …