Engineering Commons^™

Detection Of Fecal Bacteria In Watersheds Impacted By Acid Mine Drainage, Claire Addison Mcdonald

Graduate Theses, Dissertations, and Problem Reports

Escherichia coli, a fecal coliform, is an essential component of water quality monitoring, entering waterways through sewage deposits, agricultural land and livestock, and wildlife. Various fast and user-friendly methods for quantifying E. coli make it the principal indicator bacteria for fecal contamination. The most common methods for E. coli quantification are culture-based and involve cultivating samples onto nutrient agar and counting the visible colonies. However, E. coli can enter a viable but non-culturable (VBNC) state in stressful environments. Bacteria in a VBNC state are still alive but reduce key metabolic activities that typically encourage growth and division, allowing them …