Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Engineering
In Vitro Characterization Of Transforming Growth Factor-Ss1-Loaded Composites Of Biodegradable Polymer And Mesenchymal Cells, S. B. Nicoll, A. E. Denker, R. S. Tuan
In Vitro Characterization Of Transforming Growth Factor-Ss1-Loaded Composites Of Biodegradable Polymer And Mesenchymal Cells, S. B. Nicoll, A. E. Denker, R. S. Tuan
Cells and Materials
A porous, biodegradable polymer composite was fabricated from poly(L-lactic acid) (PLLA) using a solvent- casting particulate-leaching technique, and loaded with recombinant human transforming growth factor ß-1 (TGF-ß1). Tissue culture polystyrene (TCPS) and PLLA disks loaded with bovine serum albumin (BSA) served as controls. Polymer specimens were seeded at high cell density with C3H10T1/2 cells, a mouse embryonic cell line capable of differentiating into various connective tissue cell types. The polymer matrices degraded slowly in physiological saline over four weeks, with a sustained yet sporadic release of active TGF-ß1; a more controlled release of TGF-ß1 was observed in serum-containing culture medium …
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cells and Materials
This study addresses the mechanism of the chirally-restricted, ROD-independent adhesion of A6 epithelial cells to the {011} faces of calcium {R,R)-tartrate tetrahydrate crystals. The extensive and rapid adhesion of the cells to these surfaces, in the presence or absence of serum proteins, is distinctly different from the extracellular matrix-mediated adhesion to conventional tissue culture surfaces or to the {101} faces of the same crystals. The differences are manifested by insensitivity to ATP depletion, to disruption of microfilaments and microtubules and even to formaldehyde fixation of the cells. Furthermore, trypsin pretreatment does not affect cell attachment to the {011} faces, nor …