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Full-Text Articles in Engineering
Use Of Surface-Enhanced Raman Scattering (Sers) Probes To Detect Fatty Acid Receptor Activity In A Microfluidic Device, Han Zhang, Wei Zhang, Lifu Xiao, Yan Liu, Timothy A. Gilbertson, Anhong Zhou
Use Of Surface-Enhanced Raman Scattering (Sers) Probes To Detect Fatty Acid Receptor Activity In A Microfluidic Device, Han Zhang, Wei Zhang, Lifu Xiao, Yan Liu, Timothy A. Gilbertson, Anhong Zhou
Biological Engineering Faculty Publications
In this study, 4-mercaptobenzoic acid (MBA)-Au nanorods conjugated with a GPR120 antibody were developed as a highly sensitive surface-enhanced Raman spectroscopy (SERS) probe, and were applied to detect the interaction of fatty acids (FA) and their cognate receptor, GPR120, on the surface of human embryonic kidney cells (HEK293-GPRR120) cultured in a polydimethylsiloxane (PDMS) microfluidic device. Importantly, the two dominant characteristic SERS peaks of the Raman reporter molecule MBA, 1078 cm−1 and 1581 cm−1, do not overlap with the main Raman peaks from the PDMS substrate when the appropriate spectral scanning range is selected, which effectively avoided the …
The Use Of Microfluidics And Dielectrophoresis For Separation, Concentration, And Identification Of Bacteria, Cynthia Hanson, Michael Sieverts, Karen Tew, Annelise Dykes, Michaela Salisbury, Elizabeth Vargis
The Use Of Microfluidics And Dielectrophoresis For Separation, Concentration, And Identification Of Bacteria, Cynthia Hanson, Michael Sieverts, Karen Tew, Annelise Dykes, Michaela Salisbury, Elizabeth Vargis
Biological Engineering Faculty Publications
Traditional bacterial identification methods take one to two days to complete, relying on large bacteria colonies for visual identification. In order to decrease this analysis time in a cost-effective manner, a method to sort and concentrate bacteria based on the bacteria's characteristics itself is needed. One example of such a method is dielectrophoresis, which has been used by researchers to separate bacteria from sample debris and sort bacteria according to species. This work presents variations in which dielectrophoresis can be performed and their associated drawbacks and benefits specifically to bacterial identification. In addition, a potential microfluidic design will be discussed.