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Full-Text Articles in Engineering
Nmr Spectroscopy Analysis Reveals Differential Metabolic Responses In Arabidopsis Roots And Leaves Treated With A Cytokinesis Inhibitor, Thomas E. Wilkop, Minmin Wang, Angelo Heringer, Jaideep Singh, Florence Zakharov, Viswanathan V. Krishnan, Georgia Drakakaki
Nmr Spectroscopy Analysis Reveals Differential Metabolic Responses In Arabidopsis Roots And Leaves Treated With A Cytokinesis Inhibitor, Thomas E. Wilkop, Minmin Wang, Angelo Heringer, Jaideep Singh, Florence Zakharov, Viswanathan V. Krishnan, Georgia Drakakaki
Physiology Faculty Publications
In plant cytokinesis, de novo formation of a cell plate evolving into the new cell wall partitions the cytoplasm of the dividing cell. In our earlier chemical genomics studies, we identified and characterized the small molecule endosidin-7, that specifically inhibits callose deposition at the cell plate, arresting late-stage cytokinesis in arabidopsis. Endosidin-7 has emerged as a very valuable tool for dissecting this essential plant process. To gain insights regarding its mode of action and the effects of cytokinesis inhibition on the overall plant response, we investigated the effect of endosidin-7 through a nuclear magnetic resonance spectroscopy (NMR) metabolomics approach. In …
Near Simultaneous Laser Scanning Confocal And Atomic Force Microscopy (Conpokal) On Live Cells, Joree N. Sandin, Surya P. Aryal, Thomas E. Wilkop, Christopher I. Richards, Martha E. Grady
Near Simultaneous Laser Scanning Confocal And Atomic Force Microscopy (Conpokal) On Live Cells, Joree N. Sandin, Surya P. Aryal, Thomas E. Wilkop, Christopher I. Richards, Martha E. Grady
Physiology Faculty Publications
Techniques available for micro- and nano-scale mechanical characterization have exploded in the last few decades. From further development of the scanning and transmission electron microscope, to the invention of atomic force microscopy, and advances in fluorescent imaging, there have been substantial gains in technologies that enable the study of small materials. Conpokal is a portmanteau that combines confocal microscopy with atomic force microscopy (AFM), where a probe "pokes" the surface. Although each technique is extremely effective for the qualitative and/or quantitative image collection on their own, Conpokal provides the capability to test with blended fluorescence imaging and mechanical characterization. Designed …