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Articles 1 - 4 of 4
Full-Text Articles in Engineering
Exploring The Role Of Nanoparticles In Enhancing Mechanical Properties Of Hydrogel Nanocomposites, Josergio Zaragoza, Scott Fukuoka, Marcus Kraus, James Thomin, Prashanth Asuri
Exploring The Role Of Nanoparticles In Enhancing Mechanical Properties Of Hydrogel Nanocomposites, Josergio Zaragoza, Scott Fukuoka, Marcus Kraus, James Thomin, Prashanth Asuri
Bioengineering
Over the past few decades, research studies have established that the mechanical properties of hydrogels can be largely impacted by the addition of nanoparticles. However, the exact mechanisms behind such enhancements are not yet fully understood. To further explore the role of nanoparticles on the enhanced mechanical properties of hydrogel nanocomposites, we used chemically crosslinked polyacrylamide hydrogels incorporating silica nanoparticles as the model system. Rheological measurements indicate that nanoparticle-mediated increases in hydrogel elastic modulus can exceed the maximum modulus that can be obtained through purely chemical crosslinking. Moreover, the data reveal that nanoparticle, monomer, and chemical crosslinker concentrations can all …
Three-Dimensional (3d) Printed Microneedles For Microencapsulated Cell Extrusion, Chantell Farias, Roman Lyman, Cecilia Hemingway, Huong Chau, Anne Mahacek, Evangelia Bouzos, Maryam Mobed-Miremadi
Three-Dimensional (3d) Printed Microneedles For Microencapsulated Cell Extrusion, Chantell Farias, Roman Lyman, Cecilia Hemingway, Huong Chau, Anne Mahacek, Evangelia Bouzos, Maryam Mobed-Miremadi
Bioengineering
Cell-hydrogel based therapies offer great promise for wound healing. The specific aim of this study was to assess the viability of human hepatocellular carcinoma (HepG2) cells immobilized in atomized alginate capsules (3.5% (w/v) alginate, d = 225 µm ± 24.5 µm) post-extrusion through a three-dimensional (3D) printed methacrylate-based custom hollow microneedle assembly (circular array of 13 conical frusta) fabricated using stereolithography. With a jetting reliability of 80%, the solvent-sterilized device with a root mean square roughness of 158 nm at the extrusion nozzle tip (d = 325 μm) was operated at a flowrate of 12 mL/min. There was no significant …
Influence Of Macromolecular Crowding And Confinement On Enzyme Activity And Structure Under Native And Denaturing Conditions, Steven Long, Jeffrey Kunkel, Prashanth Asuri
Influence Of Macromolecular Crowding And Confinement On Enzyme Activity And Structure Under Native And Denaturing Conditions, Steven Long, Jeffrey Kunkel, Prashanth Asuri
Bioengineering
It is now well appreciated that both crowding and confinement influence enzyme structure and function due to excluded volume effects; however, the relative efficacies of these environments on protein fates remain unclear due to a lack of direct comparison studies. In this study, we explore the use of the biopolymer alginate to develop an in vitro platform to investigate the effects of both crowding and confinement on the behavior of two model enzymes - horseradish peroxidase and β-galactosidase. Alginate, in its solution phase, can be used as a crowding agent and, in its gel phase by crosslinking using divalent cations, …
Machine Learning Provides Predictive Analysis Into Silver Nanoparticle Protein Corona Formation From Physicochemical Properties, Matthew R. Findlay, Daniel N. Freitas, Maryam Mobed-Miremadi, Korin E. Wheeler
Machine Learning Provides Predictive Analysis Into Silver Nanoparticle Protein Corona Formation From Physicochemical Properties, Matthew R. Findlay, Daniel N. Freitas, Maryam Mobed-Miremadi, Korin E. Wheeler
Bioengineering
Proteins encountered in biological and environmental systems bind to engineered nanomaterials (ENMs) to form a protein corona (PC) that alters the surface chemistry, reactivity, and fate of the ENMs. Complexities such as the diversity of the PC and variation with ENM properties and reaction conditions make the PC population difficult to predict. Here, we support the development of predictive models for PC populations by relating biophysicochemical characteristics of proteins, ENMs, and solution conditions to PC formation using random forest classification. The resulting model offers a predictive analysis into the population of PC proteins in Ag ENM systems of various ENM …