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Two Dimensional Diffusional Model For Leakage Of Macromolecules In The Interstitial Space, Suthiluk Dumrongsiri

Theses

Two dimensional diffusion of macromolecules through the interstitial in the hamster cheek pouch was studied with intravital fluorescence microscopy. After topical application of Bradykinin, the movement of Fluorescein isothiocyanate (FITC)-labeled dextrans of average molecular weight 150,000 daltons (Dextran 150) was recorded on videotape which latter was played back frame-by-frame for analysis by digital image processing. Leakage of macromolecules in response to topical application of Bradykinin occurs at discrete leakage site in post caplillary venules of 15-25 um diameter, rather than in the capillary themselves. A mathematical model of two dimensional convection plus diffusion was used to simulate the macromolecule transport. …