Engineering Commons^™

Bacterial Characterization Of Louisiana Groundwater Contaminated By Dnapl-Containing Chloroethanes And Other Solvents, Kimberly Bowman

LSU Doctoral Dissertations

In support of an effort to determine the feasibility of using an in-situ bioremediation strategy for cleanup of groundwater in an area containing chlorinated solvents present as a dense non-aqueous phase liquid (DNAPL), the bacterial population in the groundwater at a Superfund site located near Baton Rouge, Louisiana was characterized. More than 3¡Á107 cells/mL in the groundwater were observed via microscopy. Universal bacterial and ¡°Dehalococcoides¡±-specific 16S rRNA gene libraries were constructed and analyzed. Universal clones grouped into 18 operational taxonomic units (OTUs), as defined by sequence similarity ¡Ý97.0%, which included several as yet undescribed organisms. Multiple unique sequences closely related …