Engineering Commons^™

Isolation And Characterization Of Bacteria In A Toluene-Producing Enrichment Culture Derived From Contaminated Groundwater At A Louisiana Superfund Site, Madison Mikes

LSU Master's Theses

In an effort to better understand the role that various microbes may play in toluene production, bacteria from a toluene-producing enrichment culture derived from contaminated groundwater at a Superfund site were cultivated and isolated on low nutrient solid media. A total of 14 solid medium formulations containing varying pH ranges, carbon sources, solidifying agents, and incubation gas headspaces were used to obtain 278 isolates in pure culture. Isolated bacteria, identified using partial 16S rRNA gene sequences, were most closely related with the genera Anoxybacillus, Azospira, Bacillus, Bradyrhizobium, Cellulosimicrobium, Micrococcus, and Propionicimonas.

Further attempts …

Contextual Adjustment Of The Ite Trip Generation Rates Using Wi-Fi And Bluetooth Technologies, Saba Doulabi

LSU Master's Theses

The ITE Trip Generation Handbook has been in common use for about half a century to estimate vehicle trips generated by more than 172 land use categories as a function of establishment size (floor area) only. However, observed trip rates display a huge error range across different sites. Although contextual adjustment factors can ameliorate the error in the ITE trip generation estimates considerably, local site-specific trip generation rates should be collected for this purpose. Due to the huge time and monetary costs of data collection, adjusting the ITE trip generation rate is ignored by many jurisdictions. The primary contribution of …