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Full-Text Articles in Engineering
Empowering Manufacturing Decisions Through Process Simulation Models, Tiffany Rau, Terence Davidovits, Rick Stock
Empowering Manufacturing Decisions Through Process Simulation Models, Tiffany Rau, Terence Davidovits, Rick Stock
Cell Culture Engineering XVI
The need for Clinical and Commercial manufacturing capacity continues to increase and at the same time there are “shortages” of production slots and time pressures. Whether one is a virtual, small or large company there are many considerations that go into the decision of how and where to manufacture the biopharmaceutical, cell therapy or vaccine. In this presentation, we explore approaches for using commercially available modeling tools, such as Super ProDesigner® and BioSolve™, to simulate the manufacturing of a “typical” monoclonal antibody product and a multivalent conjugate vaccine product. Through these hypothetical case studies, we will illustrate several important concepts …
Bioprocess Intensification And Optimisation Using Macroscopic Predictive Models Of Cell Culture Processes, Bassem Ben Yahia, Boris Fessler, Gwendal Gränicher, An-Vy Tran, Mareike Harmsen, Elmar Heinzle
Bioprocess Intensification And Optimisation Using Macroscopic Predictive Models Of Cell Culture Processes, Bassem Ben Yahia, Boris Fessler, Gwendal Gränicher, An-Vy Tran, Mareike Harmsen, Elmar Heinzle
Integrated Continuous Biomanufacturing III
Chinese Ovary Hamster (CHO) are an indispensable tool for biotechnological production of biologics which is a multi-million business. Recently, the pharmaceutical industry is increasingly focusing in the use of perfusion mode. Nevertheless, the optimal perfusion rate during biopharmaceutical perfusion production is dependent on cell metabolism which can be characterized by mathematical models. This study provides insights into the predictive capacities of systematic and simple cell modeling approaches of metabolism, growth and production of monoclonal antibodies (mAb) [1] to optimized medium composition and perfusion rate during CHO perfusion culture. We applied the metabolic steady state concept and used a segmented linear …
Bioprocess Intensification And Optimisation Using Macroscopic Predictive Models Of Cell Culture Processes, Bassem Ben Yahia, Mareike Harmsen, An-Vy Tran, Gwendal Gränicher, Boris Fessler, Elmar Heinzle
Bioprocess Intensification And Optimisation Using Macroscopic Predictive Models Of Cell Culture Processes, Bassem Ben Yahia, Mareike Harmsen, An-Vy Tran, Gwendal Gränicher, Boris Fessler, Elmar Heinzle
Integrated Continuous Biomanufacturing III
CHO cells, modeling, perfusion, ATF, segmented linear model
A Community Genome-Scale Model Of Chinese Hamster Ovary Cell Metabolism Identifies Differences In The Efficiency Of Resource Utilization For Various Bioprocesses, Hooman Hefzi
Cell Culture Engineering XV
Genome-scale models of metabolism have successfully been employed in many microbial and eukaryotic metabolic engineering efforts by guiding pathway engineering and media optimization. They have also been used to explore the genotype-phenotype relationship in mammalian cells. The publication of the genomic sequence for Chinese hamster ovary (CHO) cells has allowed generation of genome-scale metabolic models (GeMs) for this organism. Here we have developed a high-quality community CHO GeM via careful reconciliation and manual curation of three independently developed CHO GeMs. This metabolic model, consisting of over 4000 metabolites and 6000 reactions, is capable of integrating proteomic, transcriptomic, and metabolomic data …
Multi-Omic Modeling Of Translational Efficiency For Synthetic Gene Design, Joseph Longworth, Javier Gonzalez, Paul Dobson, Josselin Noirel, Neil Lawrence
Multi-Omic Modeling Of Translational Efficiency For Synthetic Gene Design, Joseph Longworth, Javier Gonzalez, Paul Dobson, Josselin Noirel, Neil Lawrence
Cell Culture Engineering XV
Controlled expression of recombinant genes in CHO cells for advanced cell engineering will require precise, coordinated control of the synthetic processes that underpin the production of specific recombinant products or the optimal stoichiometry of functional effector proteins for multigene engineering applications. Although control of recombinant gene transcription in CHO host cells is now possible, technologies that enable control of recombinant mRNA translation rate are lacking. This is undesirable as in eukaryotic cells, cellular mRNA concentration itself may only explain a relatively small proportion of the variation in cellular protein abundance; mRNA translation rate is by far the most important contributor …
Thermodynamics Of Migration In Polymers: Can Contamination From Materials In Contact Be Predicted, Controlled And Possibly Avoided?, Olivier Vitrac, Phuong Nguyen
Thermodynamics Of Migration In Polymers: Can Contamination From Materials In Contact Be Predicted, Controlled And Possibly Avoided?, Olivier Vitrac, Phuong Nguyen
Single-Use Technologies: Bridging Polymer Science to Biotechnology Applications
The contamination from materials is coined as “migration” and refers to a combination of various mass transfer mechanisms under thermodynamic control: molecular or mutual diffusion, sorption and desorption phenomena linear with concentration or not. The contamination pathways are particularly complicated when they involve several components or steps and strong interactions between materials and the medium in contact. They have been studied and mathematically described with two possible purposes: i) simplified approaches with a goal of overestimating the real contamination and ii) more sophisticated approaches to optimize the design of polymer materials, packaging materials or to redesign plastic additives with lower …
Numerical Computation Of Hydrodynamic Behavior Of Biomass Particles In Circulating Fluidized Beds, Afsin Gungor Dr.
Numerical Computation Of Hydrodynamic Behavior Of Biomass Particles In Circulating Fluidized Beds, Afsin Gungor Dr.
The 13th International Conference on Fluidization - New Paradigm in Fluidization Engineering
A model using a particle based approach is developed to accurately predict the hydrodynamic behavior of biomass particles in CFBs. Generally, the change in the pressure gradient with height in CFB riser is small. Numerical results are in good agreement with experiments, both in form and magnitude.
Modeling On Heterogeneous Structure In Acceleration Regime Of Gas-Solid Riser Flows, Chao Zhu, Jun You, Dawei Wang, Liang-Shih Fan
Modeling On Heterogeneous Structure In Acceleration Regime Of Gas-Solid Riser Flows, Chao Zhu, Jun You, Dawei Wang, Liang-Shih Fan
The 12th International Conference on Fluidization - New Horizons in Fluidization Engineering
Recent measurement of solid concentration in gas-solid riser flows by Electric Capacitance Tomography (ECT) reveals a strong heterogeneous structure, typically represented by a core-annulus-wall zone pattern. In this paper we present a mechanistic model in which the formation of the heterogeneous structure is due to the radial migration of solid flow from the wall toward center as well as due to the non-uniform acceleration of solids across the cross-section near the bottom of the riser. Firstly we present the general governing equations and discuss problem closure; then a simplified model with one-way flow coupling between the wall region and the …
Modeling Fuel Mixing In A Fluidized Bed Combustor, David Pallarès, Filip Johnsson
Modeling Fuel Mixing In A Fluidized Bed Combustor, David Pallarès, Filip Johnsson
The 12th International Conference on Fluidization - New Horizons in Fluidization Engineering
This paper presents a 3-dimensional model for fuel mixing in fluidized bed combustors. The model accounts for the mixing patterns experimentally shown to govern the mixing in the different zones of the riser and the return leg and can be applied both under bubbling and circulating regimes. Thus, the semi-empirical basis of the model was previously validated in different large-scale fluidized bed combustors and is combined with a model for fuel particle conversion to obtain the fuel concentration. Results obtained with the model are compared with experimental data from the Chalmers 12 MWth CFB combustor, yielding reasonably agreement.
Modeling Of An Interconnected Fluidized Bed Reactor For Chemical Looping Combustion, Min Xu, Naoko Ellis, Ho-Jung Ryu , C. Jim Lim
Modeling Of An Interconnected Fluidized Bed Reactor For Chemical Looping Combustion, Min Xu, Naoko Ellis, Ho-Jung Ryu , C. Jim Lim
The 12th International Conference on Fluidization - New Horizons in Fluidization Engineering
Chemical Looping Combustion (CLC) is comprised of two reactors, in which direct contact between air and fuel is avoided. A metal oxide, as oxygen carrier, transports oxygen from the air reactor to the fuel reactor while circulating between them. Based on the hydrodynamics coupled with reaction kinetics of oxygen carrier from the literature, a model for an interconnected fluidized bed reactor has been developed to optimize the design and operation of the reactor system. The model considers the chemical reaction of a single particle and a particle population balance for the calculation of bed particle conversion. The core-annulus and two-phase …
Simulation Of A Silicon Cvd Spouted Fluidized Bed Reactor: Semi-Batch Operations, Juliana Piña, Verónica Bucalá, Susana N. Schbib, Paul Ege, Hugo Ignacio De Lasa
Simulation Of A Silicon Cvd Spouted Fluidized Bed Reactor: Semi-Batch Operations, Juliana Piña, Verónica Bucalá, Susana N. Schbib, Paul Ege, Hugo Ignacio De Lasa
The 12th International Conference on Fluidization - New Horizons in Fluidization Engineering
A comprehensive multiphase gas-solid mathematical model that successfully describes the batch growth of silicon particles in a chemical vapor deposition (CVD) submerged spouted bed reactor is extended to simulate semi-batch operations with periodic seeds additions and product extractions. This model takes into account the fluidized bed reactor as well as a population balance equation representing particle growth and agglomeration. Experimental data obtained from semi-batch operation in a pilot scale reactor at REC Silicon Inc. are used to evaluate the proposed mathematical model.