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Full-Text Articles in Engineering
Characterization And Optimization Of A Chromatographic Process Based On Ethylenediamine-N,N,N′,N′-Tetra(Methylphosphonic) Acid-Modified Zirconia Particles, Sabyasachi Sarkar, Peter W. Carr, Clayton V. Mcneff, Anuradha Subramanian
Characterization And Optimization Of A Chromatographic Process Based On Ethylenediamine-N,N,N′,N′-Tetra(Methylphosphonic) Acid-Modified Zirconia Particles, Sabyasachi Sarkar, Peter W. Carr, Clayton V. Mcneff, Anuradha Subramanian
Papers in Biotechnology
The primary objective of work was to characterize, optimize and model a chromatographic process based on ethylenediamine-N,N,N′,N′-tetra(methylphosphonic) acid (EDTPA)-modified zirconia particles. Zirconia particles were produced by spray-drying colloidal zirconia. Zirconia spheres produced were further classified, calcined and modified with EDTPA to yield a solid-phase support for use in bio-chromatography (r_PEZ). Specifically, the ability of r_PEZ to selectively bind and enrich IgG, IgA, and IgM from biological fluids was evaluated and demonstrated. To better understand the force of interaction between the IgG and the r_PEZ, the equilibrium disassociation constant (Kd) was determined by static binding isotherms, as a function of temperature …