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Full-Text Articles in Engineering
Cip2a Immunosensor Comprised Of Vertically-Aligned Carbon Nanotube Interdigitated Electrodes Towards Point-Of-Care Oral Cancer Screening, Shaowei Ding, Suprem R. Das, Benjamin J. Brownlee, Kshama Parate, Taylor Davis, Loreen R. Stromberg, Edward K.L. Chan, Joseph Katz, Brian D. Iverson, Jonathan C. Claussen
Cip2a Immunosensor Comprised Of Vertically-Aligned Carbon Nanotube Interdigitated Electrodes Towards Point-Of-Care Oral Cancer Screening, Shaowei Ding, Suprem R. Das, Benjamin J. Brownlee, Kshama Parate, Taylor Davis, Loreen R. Stromberg, Edward K.L. Chan, Joseph Katz, Brian D. Iverson, Jonathan C. Claussen
Faculty Publications
Vertically aligned carbon nanotube array (VANTA) coatings have recently garnered much attention due in part to their unique material properties including light absorption, chemical inertness, and electrical conductivity. Herein we report the first use of VANTAs grown via chemical vapor deposition in a 2D interdigitated electrode (IDE) footprint with a high height-to-width aspect ratio (3:1 or 75:25 µm). The VANTA-IDE is functionalized with an antibody (Ab) specific to the human cancerous inhibitor PP2A (CIP2A)—a alivary oncoprotein that is associated with a variety of malignancies such as oral, breast, and multiple myeloma cancers. The resultant immunosensor is capable of detecting CIP2A …
Optimum 3d Matrix Stiffness For Maintenance Of Cancer Stem Cells Is Dependent On Tissue Origin Of Cancer Cells, Esmaiel Jabbari, Samaneh K. Sarvestani, Leily Daneshian, Seyedsina Moeinzadeh
Optimum 3d Matrix Stiffness For Maintenance Of Cancer Stem Cells Is Dependent On Tissue Origin Of Cancer Cells, Esmaiel Jabbari, Samaneh K. Sarvestani, Leily Daneshian, Seyedsina Moeinzadeh
Faculty Publications
Introduction
The growth and expression of cancer stem cells (CSCs) depend on many factors in the tumor microenvironment. The objective of this work was to investigate the effect of cancer cells’ tissue origin on the optimum matrix stiffness for CSC growth and marker expression in a model polyethylene glycol diacrylate (PEGDA) hydrogel without the interference of other factors in the microenvironment.
Methods
Human MCF7 and MDA-MB-231 breast carcinoma, HCT116 colorectal and AGS gastric carcinoma, and U2OS osteosarcoma cells were used. The cells were encapsulated in PEGDA gels with compressive moduli in the 2-70 kPa range and optimized cell seeding density …
Three-Dimensional Engineered Matrix To Study Cancer Stem Cells And Tumorsphere Formation: Effect Of Matrix Modulus, Xiaoming Yang, Samaneh Kamali Sarvestani, Seyedsina Moeinzadeh, Xuezhong He, Esmaiel Jabbari
Three-Dimensional Engineered Matrix To Study Cancer Stem Cells And Tumorsphere Formation: Effect Of Matrix Modulus, Xiaoming Yang, Samaneh Kamali Sarvestani, Seyedsina Moeinzadeh, Xuezhong He, Esmaiel Jabbari
Faculty Publications
Maintenance of cancer stem cells (CSCs) is regulated by the tumor microenvironment. Synthetic hydrogels provide the flexibility to design three-dimensional (3D) matrices to isolate and study individual factors in the tumor microenvironment. The objective of this work was to investigate the effect of matrix modulus on tumorsphere formation by breast cancer cells and maintenance of CSCs in an inert microenvironment without the interference of other factors. In that regard, 4T1 mouse breast cancer cells were encapsulated in inert polyethylene glycol diacrylate hydrogels and the effect of matrix modulus on tumorsphere formation and expression of CSC markers was investigated. The gel …