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Clinically Amendable, Defined, And Rapid Induction Of Human Brain Organoids From Induced Pluripotent Stem Cells, Eva Tomaskovic-Crook, Jeremy Micah Crook
Clinically Amendable, Defined, And Rapid Induction Of Human Brain Organoids From Induced Pluripotent Stem Cells, Eva Tomaskovic-Crook, Jeremy Micah Crook
Australian Institute for Innovative Materials - Papers
Human brain organoids provide opportunities to produce three-dimensional (3D) brain-like tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a protocol for rapid and defined induction of brain organoids from human induced pluripotent stem cells (iPSCs), using commercially available culture and differentiation media and a cheap, easy to handle and clinically approved semisynthetic hydrogel. Importantly, the methodology is uncomplicated, well-defined, and reliable for reproducible and scalable organoid generation, and amendable to principles of current good laboratory practice (cGLP), with the potential for prospective adaptation to current good manufacturing practice (cGMP) toward clinical compliance.
Brain On A Bench Top, Rodrigo Lozano, Leo Stevens, Brianna C. Thompson, Kerry J. Gilmore, Robert A. Gorkin Iii, Elise M. Stewart, Marc In Het Panhuis, Mario I. Romero-Ortega, Gordon G. Wallace
Brain On A Bench Top, Rodrigo Lozano, Leo Stevens, Brianna C. Thompson, Kerry J. Gilmore, Robert A. Gorkin Iii, Elise M. Stewart, Marc In Het Panhuis, Mario I. Romero-Ortega, Gordon G. Wallace
Australian Institute for Innovative Materials - Papers
Tissue grafting and organ transplantation are common techniques in modern medicine, but suffer from the widely-reported limitations of constrained supply and the risk of rejection, necessitating a lifetime of immunosuppressant medication [1]. The field of tissue engineering has advanced markedly over the last decade, and engineered tissues are under development for both regenerative medicine applications as well as the formation of accurate in vitro models for the study of fundamental tissue behaviours, disease states and drug discovery. Both biological and synthetic materials have been formed into cell-supporting tissue scaffolds [2,3]. In broad terms, biological materials and particularly extracellular matrix (ECM) …
Pre-Differentiation Of Human Neural Stem Cells Into Gabaergic Neurons Prior To Transplant Results In Greater Repopulation Of The Damaged Brain And Accelerates Functional Recovery After Transient Ischemic Stroke, Hima C. S Abeysinghe, Laita Bokhari, Anita F. Quigley, Mahesh A. Choolani, Jerry Chan, Gregory J. Dusting, Jeremy M. Crook, Nao R. Kobayashi, Carli Roulston
Pre-Differentiation Of Human Neural Stem Cells Into Gabaergic Neurons Prior To Transplant Results In Greater Repopulation Of The Damaged Brain And Accelerates Functional Recovery After Transient Ischemic Stroke, Hima C. S Abeysinghe, Laita Bokhari, Anita F. Quigley, Mahesh A. Choolani, Jerry Chan, Gregory J. Dusting, Jeremy M. Crook, Nao R. Kobayashi, Carli Roulston
Australian Institute for Innovative Materials - Papers
2015 Abeysinghe et al. Introduction: Despite attempts to prevent brain injury during the hyperacute phase of stroke, most sufferers end up with significant neuronal loss and functional deficits. The use of cell-based therapies to recover the injured brain offers new hope. In the current study, we employed human neural stem cells (hNSCs) isolated from subventricular zone (SVZ), and directed their differentiation into GABAergic neurons followed by transplantation to ischemic brain. Methods: Pre-differentiated GABAergic neurons, undifferentiated SVZ-hNSCs or media alone were stereotaxically transplanted into the rat brain (n=7/group) 7 days after endothelin-1 induced stroke. Neurological outcome was assessed by neurological deficit …