Engineering Commons^™

Utilizing Fluorescent Nanoscale Particles To Create A Map Of The Electric Double Layer, Quintus Owen

Theses and Dissertations

The interactions between charged particles in solution and an applied electric field follow several models, most notably the Gouy-Chapman-Stern model, for the establishment of an electric double layer along the electrode, but these models make several assumptions of ionic concentrations and an infinite bulk solution. As more scientific progress is made for the finite and single molecule reactions inside microfluidic cells, the limitations of the models become more extreme. Thus, creating an accurate map of the precise response of charged nanoparticles in an electric field becomes increasingly vital. Another compounding factor is Brownian motion’s inverse relationship with size: large easily …

Investigation Of Dielectrophoretic Microfluidic Trap System For Separation And Parallel Analysis Of Single Particles, Tae Joon Kwak

Theses and Dissertations

Separation and identification of single molecules and particles based on their chemical, biochemical and physical properties are critical in wide range of biomedical applications. Manipulating a single biomolecule requires sensitive approaches to avoid damage to the molecule. Recent progress in micro- and nano-technology enabled the development of various novel methods and devices to trap, separate, and characterize micro- and nano-particles. In this dissertation, a microfluidic particle trap system to electrically separate particles at the single particle level was developed through particle manipulation methods using dielectrophoresis. The research in this dissertation will explain the operation strategy and setup of the novel …

Effect Of Junction Geometry On Monodispersed Microdroplet Generation In Microfluidic Aqueous Two-Phase Systems, Young Gyu Nam

Theses and Dissertations

Aqueous two-phase system (ATPS) consists of two immiscible water-based solutions of polymers, which can form phase partitioning. Dextran and polyethylene glycol I used in this thesis is the one of common components of aqueous two-phase system give a reliable and incompatible environment for purification of biomedical products and cellular macromolecules. Recently, ATPS have received increasing attention as a separation method in microfluidic device due to the advantages of biocompatibility, unlimited combination, and low interfacial tension. Hence, it became an important to discover researches related to ATPS microfluidic device.

Microdroplets produced in microfluidic device are a largely interesting phenomenon for various …