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Full-Text Articles in Engineering

The Confounding Effects Of Particle Size And Substrate Bulk Density On Phanerochaete Chrysosporium Pretreatment On Panicum Virgatum, Amanda N. Hickman, Sue E. Nokes, William S. Sympson, Mathew J. Ruwaya, Michael D. Montross, Barbara L. Knutson Jul 2016

The Confounding Effects Of Particle Size And Substrate Bulk Density On Phanerochaete Chrysosporium Pretreatment On Panicum Virgatum, Amanda N. Hickman, Sue E. Nokes, William S. Sympson, Mathew J. Ruwaya, Michael D. Montross, Barbara L. Knutson

Biosystems and Agricultural Engineering Faculty Publications

Phanerochaete chrysosporium treatment is less effective as a biological pretreatment on feedstock with larger particle sizes. We hypothesized that the improved effectiveness of the pretreatment when smaller particle sizes are used may be due to the inherently higher bulk density with smaller particle sizes. The effects of substrate bulk density and particle size on the efficacy of P. chrysosporium pretreatment of switchgrass (Panicum virgatum) was tested experimentally. Phanerochaete chrysosporium was grown on senesced switchgrass (2 different particle sizes) with various bulk densities. In all treatments, the fungal-pretreated samples released more glucose during enzymatic saccharification than the control sample. …


Switchgrass (Panicum Virgatum) Fermentation By Clostridium Thermocellum And Clostridium Saccharoperbutylacetonicum Sequential Culture In A Continuous Flow Reactor, Noelia M. Elia, Sue E. Nokes, Michael D. Flythe Jan 2016

Switchgrass (Panicum Virgatum) Fermentation By Clostridium Thermocellum And Clostridium Saccharoperbutylacetonicum Sequential Culture In A Continuous Flow Reactor, Noelia M. Elia, Sue E. Nokes, Michael D. Flythe

Biosystems and Agricultural Engineering Faculty Publications

The study was conducted to evaluate fermentation by Clostridium thermocellum and C. saccharoperbutylacetonicum in a continuous-flow, high-solids reactor. Liquid medium was continuously flowed through switchgrass (2 mm particle size) at one of three flow rates: 83.33 mL h1 (2 L d−1), 41.66 mL h−1 (1 L d−1), and 20.833 mL h−1 (0.5 L d−1). The cellulolytic phase was initiated by culturing C. thermocellum (63 °C, 24 h). The temperature was decreased (35) and C. saccharoperbutylacetonicum was inoculated. When metabolism decreased (96 h), the temperature was increased (63 °C; 24 h) …