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Full-Text Articles in Engineering
Identification Of Developmental Stage And Anatomical Fraction Contributions To Cell Wall Recalcitrance In Switchgrass, Jacob D. Crowe, Nicholas Feringa, Sivakumar Pattathil, Brian Merritt, Cliff Foster, Dayna Dines, Rebecca G. Ong, David B. Hodge
Identification Of Developmental Stage And Anatomical Fraction Contributions To Cell Wall Recalcitrance In Switchgrass, Jacob D. Crowe, Nicholas Feringa, Sivakumar Pattathil, Brian Merritt, Cliff Foster, Dayna Dines, Rebecca G. Ong, David B. Hodge
Rebecca Ong
Background Heterogeneity within herbaceous biomass can present important challenges for processing feedstocks to cellulosic biofuels. Alterations to cell wall composition and organization during plant growth represent major contributions to heterogeneity within a single species or cultivar. To address this challenge, the focus of this study was to characterize the relationship between composition and properties of the plant cell wall and cell wall response to deconstruction by NaOH pretreatment and enzymatic hydrolysis for anatomical fractions (stem internodes, leaf sheaths, and leaf blades) within switchgrass at various tissue maturities as assessed by differing internode. Results Substantial differences in both cell wall composition …
Strategies For The Production Of Cell Wall-Deconstructing Enzymes In Lignocellulosic Biomass And Their Utilization For Biofuel Production, Sang-Hyuck Park, Rebecca Garlock Ong, Mariam Sticklen
Strategies For The Production Of Cell Wall-Deconstructing Enzymes In Lignocellulosic Biomass And Their Utilization For Biofuel Production, Sang-Hyuck Park, Rebecca Garlock Ong, Mariam Sticklen
Rebecca Ong
Microbial cell wall-deconstructing enzymes are widely used in the food, wine, pulp and paper, textile, and detergent industries and will be heavily utilized by cellulosic biorefineries in the production of fuels and chemicals. Due to their ability to use freely available solar energy, genetically engineered bioenergy crops provide an attractive alternative to microbial bioreactors for the production of cell wall-deconstructing enzymes. This review article summarizes the efforts made within the last decade on the production of cell wall-deconstructing enzymes in planta for use in the deconstruction of lignocellulosic biomass. A number of strategies have been employed to increase enzyme yields …
Inhibition Of Microbial Biofuel Production In Drought-Stressed Switchgrass Hydrolysate, Rebecca Garlock Ong, Alan Higbee, Scott Bottoms, Quinn Dickinson, Dan Xie, Scott A. Smith, Jose Serate, Edward Pohlman, Arthur Daniel Jones, Joshua J. Coon, Trey S. Kato, Gregg R. Sanford, Dustin Eilert, Dustin G. Oates, Jeff S. Piotrowski, Donn M. Bates, David Cavalier, Yaoping Zhang
Inhibition Of Microbial Biofuel Production In Drought-Stressed Switchgrass Hydrolysate, Rebecca Garlock Ong, Alan Higbee, Scott Bottoms, Quinn Dickinson, Dan Xie, Scott A. Smith, Jose Serate, Edward Pohlman, Arthur Daniel Jones, Joshua J. Coon, Trey S. Kato, Gregg R. Sanford, Dustin Eilert, Dustin G. Oates, Jeff S. Piotrowski, Donn M. Bates, David Cavalier, Yaoping Zhang
Rebecca Ong
Background: Interannual variability in precipitation, particularly drought, can affect lignocellulosic crop biomass yields and composition, and is expected to increase biofuel yield variability. However, the effect of precipitation on downstream fermentation processes has never been directly characterized. In order to investigate the impact of inter-annual climate variability on biofuel production, corn stover and switchgrass were collected during 3 years with significantly different precipitation profiles, representing a major drought year (2012) and 2 years with average precipitation for the entire season (2010 and 2013). All feedstocks were AFEX (ammonia fiber expansion)-pretreated, enzymatically hydrolyzed, and the hydrolysates separately fermented using xylose-utilizing strains …