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Full-Text Articles in Engineering
Preparative Synthesis Of Dtdp-L-Rhamnose Through Combined Enzymatic Pathways, Young Bok Abraham Kang, Yung-Hun Yang, Kwang-Won Lee, Sun-Gu Lee, Jae Kyung Sohng, Hei Chan Lee, Kwangkyoung Liou, Byung-Gee Kim
Preparative Synthesis Of Dtdp-L-Rhamnose Through Combined Enzymatic Pathways, Young Bok Abraham Kang, Yung-Hun Yang, Kwang-Won Lee, Sun-Gu Lee, Jae Kyung Sohng, Hei Chan Lee, Kwangkyoung Liou, Byung-Gee Kim
Faculty Publications - Biomedical, Mechanical, and Civil Engineering
dTDP-L-rhamnose, an important precursor of O-antigen, was prepared on a large scale from dTMP by executing an one-pot reaction in which six enzymes are involved. Two enzymes, dTDP-4-keto-6-deoxy-D-glucose 3,5-epimerase and dTDP-4-keto-rhamnose reductase, responsible for the conversion of dTDP-4-keto-6-deoxy- D-glucose to dTDP-L-rhamnose, were isolated from their putative sequences in the genome of Mesorhizobium loti, functionally expressed in Escherichia coli, and their enzymatic activities were identified. The two enzymes were combined with an enzymatic process for dTDP-4- keto-6-deoxy-D-glucose involving TMP kinase, acetate kinase, dTDP-glucose synthase, and dTDP-glucose 4,6- dehydratase, which allowed us to achieve a preparative scale synthesis of dTDP-L-rhamnose using dTMP …
Characterization Of Gdp-Mannose Pyrophosphorylase From Escherichia Coli O157:H7 Edl933 And Its Broad Substrate Specificity, Yung-Hun Yang, Young Bok (Abraham) Kang, Kwang-Won Lee, Tek-Hyung Lee, Sung-Soo Park, Bum-Yeol Hwang, Byung-Gee Kim
Characterization Of Gdp-Mannose Pyrophosphorylase From Escherichia Coli O157:H7 Edl933 And Its Broad Substrate Specificity, Yung-Hun Yang, Young Bok (Abraham) Kang, Kwang-Won Lee, Tek-Hyung Lee, Sung-Soo Park, Bum-Yeol Hwang, Byung-Gee Kim
Faculty Publications - Biomedical, Mechanical, and Civil Engineering
GDP-mannose pyrophosphorylase gene (ManC) of Escherichia coli (E. coli) O157 was cloned and expressed as a highly soluble protein in E. coli BL21 (DE3). The enzyme was subsequently purified using hydrophobic and ion exchange chromatographies. ManC showed very broad substrate specificities for four nucleotides and various hexose-1-phosphates, yielding ADP-mannose, CDP-mannose, UDP-mannose, GDP-mannose, GDP-glucose and GDP-2-deoxy-glucose.
Model Predictive Control Of A Parafoil And Payload System, Nathan Slegers, Mark Costello
Model Predictive Control Of A Parafoil And Payload System, Nathan Slegers, Mark Costello
Faculty Publications - Biomedical, Mechanical, and Civil Engineering
No abstract provided.
An Existing Global Heptane Mechanism Augmented With Diffusive Transport, Michael R. Foster, Howard Pearlman
An Existing Global Heptane Mechanism Augmented With Diffusive Transport, Michael R. Foster, Howard Pearlman
Faculty Publications - Biomedical, Mechanical, and Civil Engineering
The couplings between diffusive transport and the temperature and species concentration distributions associated with low and intermediate temperature heptane oxidation are explored using an existing four-step heptane mechanism, tuned for elevated pressures. The energy and species concentration equations are augmented with diffusive fluxes for heat and species and solved numerically in a one-dimensional domain. The ignition delay times are also tabulated and compared with the zero-dimensional data reported in the literature.