Engineering Commons^™

Smart Engineering Of Various Enzymes For Asymmetric Synthesis Of Chiral Molecules On Industrial Scale, Haibin Chen, Marco Bocola, Thomas Daußmann, Yong Koy Bong

Enzyme Engineering XXV

The commercialization of sustainable enzymatic and microbial catalysis technology is gaining increasing priority for the synthesis of chiral compounds from achiral precursors that require high selectivity and high substrate load. Thus, the fast and cost-effective development of novel biocatalytic processes using an integrated platform offers significant opportunities to fine chemical, pharmaceutical, food & feed, material and related industries.

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Sustainable Biocatalytic Synthesis Of Β-Hydroxyl-Α-Amino Acids On An Industrial Scale, Haibin Chen, Haibin Chen, Yong Koy Bong, Marco Bocola, Thomas Daußmann

Enzyme Engineering XXV

The development and commercialization of sustainable enzymatic and microbial catalysis technologies is gaining increasing priority to reduce the environmental impact of chemical and related industries.

Enzymes offer, as common platform, significant opportunities for innovations to enhance production capabilities to meet these new reduced impact demands, whilst retaining product quality and keeping costs down. To enable cost efficient biocatalytic processes, however, high selectivity, high activity, high substrate loadings and tolerance to organic solvents are required. This is in general not sufficiently displayed by natural enzymes, despite providing high selectivity and activity on native substrates under physiological conditions. As a solution, enzyme …

Understanding Promiscuity In Class Ii Pyruvate Aldolases, Ulf Hanefeld, Stefan Marsden, Isabel Bento

Enzyme Engineering XXV

Aldolases are grouped based on their strict donor specificity. To expand the use of these versatile enzymes it is essential to identify aldolases that utilize different donors. Recently a number of pyruvate aldolases that accepted hydroxypyruvate was identified [1]. The class II hydroxy ketoacid aldolase A5VH82 from Sphingomonas wittichii RW1 (SwHKA) accepts hydroxypyruvate and was expressed for detailed studies. A thorough investigation including X-ray crystallography allowed new insights into the mechanistic details. This revealed a phosphate dependence of the enzyme and a preference for Mn (II) [2]. More importantly the substrate scope was studied. SwHKA is promiscuous …

Strategies And Software Tools For Engineering Protein Tunnels And Dynamical Gates, Jiri Damborsky, David Bednar, Sergio Marques, Piia Kokkonen, Gaspar Pinto, Joan Planas Iglesias, Milos Musil, Jiri Hon, Lenka Sumbalova, Ondrej Vavra, Jiri Filipovic, Bara Kozlikova, Martin Marek, Zbynek Prokop, Jan Stourac

Enzyme Engineering XXV

Improvements in the catalytic activity, substrate specificity or enantioselectivity of enzymes are traditionally achieved by modification of enzymes’ active sites. We have recently proposed that the enzyme engineering endeavors should target both the active sites and the access tunnels/channels [1,2]. Using the model enzymes haloalkane dehalogenases, we have demonstrated that engineering of access tunnels provides enzymes with significantly improved catalytic properties [3] and stability [4]. User-friendly software tools Caver [5], Caver Analyst [6], CaverDock [7] and Caver Web [8], have been developed for the computational design of protein tunnels/channels; FireProt [9] and HotSpot Wizard [10] for automated design of stabilizing …

Conference Program, Huimin Zhao, John Wong

Enzyme Engineering XXV

No abstract provided.