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Improved Single Molecule Detection Platform Using A Buried Arrow Design, Thomas Allen Wall
Improved Single Molecule Detection Platform Using A Buried Arrow Design, Thomas Allen Wall
Theses and Dissertations
As the microelectronics industry pushes microfabrication processes further, the lab-on-a-chip field has continued to piggy-back off the industry's fabrication capabilities with the goal of producing total chemical and biological systems on small chip-size platforms. One important function of such systems is the ability to perform single molecule detection. There are currently many methods being researched for performing single molecule detection, both macro and micro in scale. This dissertation focuses on an optofluidic, lab-on-a-chip platform called the ARROW biosensor, which possesses several advantages over macro-scale single molecule detection platforms. These advantages include an amplification-free detection scheme, cheap parallel fabrication techniques, rapid …
Engineering Cell-Free Protein Synthesis Technology For Codon Reassignment, Biotherapeutics Production Using Just-Add-Water System, And Biosensing Endocrine Disrupting Compounds, Sayed Mohammad Salehi
Engineering Cell-Free Protein Synthesis Technology For Codon Reassignment, Biotherapeutics Production Using Just-Add-Water System, And Biosensing Endocrine Disrupting Compounds, Sayed Mohammad Salehi
Theses and Dissertations
Cell-free protein synthesis is an emerging technology that has many applications. The open nature of this system makes it a compelling technology that can be manipulated to answer many needs that are unavailable in other systems. This dissertation reports on engineering this technology for: 1) sense codon emancipation for incorporation of multiple unnatural amino acids; 2) expressing a hard-to-express anticancer biotherapeutic and introducing a just-add-water system; 3) a biosensing ligand that interacts with nuclear hormone receptors. Emancipating sense codons toward a minimized genetic code is of significant interest to science and engineering. A promising approach to sense codon emancipation is …