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Plant Sciences

University of Nebraska - Lincoln

Department of Biological Systems Engineering: Dissertations and Theses

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Genetically Modified

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Characterization Of Genetically Modified High Biomass Producing Tobacco Plant, Pankaj Singh Kuhar Jul 2014

Characterization Of Genetically Modified High Biomass Producing Tobacco Plant, Pankaj Singh Kuhar

Department of Biological Systems Engineering: Dissertations and Theses

Global warming and peak oil has clouded our energy security. In light of this situation, bioethanol as emerged as one of the most amenable solutions to the problem. However bioethanol has its own shortcomings and transgenics seem imperative to exploit its full potential. A high biomass producing line in transgenic tobacco (Nicotiana tabacum cv. Xanthi) was identified during a routine genetic transformation, termed giant recombinant (GR). To characterize the phenotype of the giant line, growth rate and lignocellulosic composition was analyzed relative to the non-transgenic control line. The GR line accounted for 240% more biomass than the untransformed line …