Engineering Commons^™

Medical Applications Of Ultrasound: T-Cell Drug Delivery, Osteoporosis Diagnosis, And Biofilm Mitigation, Alina Karki

Graduate College Dissertations and Theses

The ability of ultrasound to localize acoustic energy deposition and induce a biological effect within a target is examined in three novel biomedical applications: sonoporation, osteoporosis diagnosis, and biofilm mitigation.Ultrasound can excite encapsulated microbubbles, causing an acoustic cavitation effect in the vicinity of cells, temporarily increasing membrane permeability, and allowing cells to uptake foreign molecules. This non-viral transfection technique is called sonoporation. Our experimental study demonstrated that it could be effective for small interfering RNA (siRNA) delivery into an isolated mouse and human T-cells, which is a complex process despite its importance in treating numerous diseases. T-cells are non-proliferating, while …