Engineering Commons^™

Phanerochaete Chrysosporium Pretreatment Of Biomass To Enhance Solvent Production In Subsequent Bacterial Solid-Substrate Cultivation, Wanying Yao, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

This study investigated pretreatment of corn stover using solid substrate cultivation (SSC) of Phanerochaete chrysosporium (P. chrysosporium) to improve subsequent accessibility to cellulose. Thereafter, Clostridium thermocellum (C. thermocellum) was directly inoculated onto the pretreated biomass to accomplish hydrolysis, followed by solventogenesis by introducing Clostridium beijerinckii (C. beijerinckii).

An enzyme suite containing laccase, lignin peroxidase and manganese peroxidase activity was detected during the cultivation of P. chrysosporium on corn stover within 288 h at an initial moisture content 𝓌_H2O = 80% (mass of water/total mass). Incubation factors, such as substrate moisture content and cultivation …

Economic Analysis Of Cellulase Production Methods For Bio-Ethanol, Jun Zhuang, Mary A. Marchant, Sue E. Nokes, Herbert J. Strobel

Biosystems and Agricultural Engineering Faculty Publications

The cost of cellulase enzymes has limited the feasibility of producing ethanol from fibrous biomass. Traditional submerged fermentation (SmF) was compared to an alternative method of producing cellulase, solid state cultivation (SSC). Results from an economic analysis indicated that the unit costs for cellulase enzyme production were $15.67 (The prices are all 2004 prices in this article, except otherwise stated. We deflated newer prices to 2004 prices using a deflation factor 0.9 per year and inflated older prices to 2004 prices using an inflation factor 1.1.) per kilogram ($/kg) and $40.36/kg, for the SSC and SmF methods, respectively, while the …