Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Keyword
-
- Cancer (2)
- Tissue engineering (2)
- 3D Printing (1)
- AMPAR (1)
- Benzene (1)
-
- Biodegradable (1)
- Biomedical engineering (1)
- Biosensors (1)
- Biotechnology (1)
- Cell culturing (1)
- Cell growth (1)
- Computational Neuroscience (1)
- Dormancy (1)
- Drug screening (1)
- Engineering (1)
- Enzyme Kinetics (1)
- Expansion Microscopy (1)
- FRET (1)
- Genetics (1)
- High content (1)
- High throughput (1)
- Immune response (1)
- Immunofluorescence (1)
- Islet cell transplantation (1)
- Learning and Memory (1)
- Localized immunosuppressive therapy (1)
- Metastasis (1)
- Microfluidics (1)
- Multi-Color (1)
- Multi-output assay (1)
Articles 1 - 18 of 18
Full-Text Articles in Engineering
A Novel High-Throughput, High-Content Three-Dimensional Assay For Determination Of Tumor Invasion And Dormancy, Mahera M. Husain, Theodore J. Puls, Sherry Voytik-Harbin
A Novel High-Throughput, High-Content Three-Dimensional Assay For Determination Of Tumor Invasion And Dormancy, Mahera M. Husain, Theodore J. Puls, Sherry Voytik-Harbin
The Summer Undergraduate Research Fellowship (SURF) Symposium
Metastasis accounts for most cancer deaths, while dormancy of tumor cells leads to unexpected cancer recurrence. These two aspects of cancer remain relatively untreatable in part because current two-dimensional (2D) methods of high-throughput drug screening cannot quantify outcomes related to these phenotypes. Three-dimensional (3D) in-vitro tumor models are a promising alternative because they better recreate the tumor microenvironment and relevant phenotypes. However, outcome measures for high-throughput screening of these systems are often limited to single measures such as metabolic activity using assays that are not standardized or optimized for 3D models. To address this gap, the objective of this work …
Localized Immunosuppression Therapy For Islet Cell Encapsulation, Madeline Mclaughlin, Clarissa Stephens, Sherry Voytik-Harbin
Localized Immunosuppression Therapy For Islet Cell Encapsulation, Madeline Mclaughlin, Clarissa Stephens, Sherry Voytik-Harbin
The Summer Undergraduate Research Fellowship (SURF) Symposium
Type 1 diabetes, an autoimmune disease in which the body’s immune system destroys the insulin-producing beta cells necessary for managing a person’s blood glucose levels, affects 1.25 million Americans. A potential treatment for this disease is islet cell transplantation where Islets of Langerhans, containing the beta cells, are transplanted from a normal donor to a diabetic recipient to regulate blood glucose levels and provide insulin independence. Similar to whole organ transplantation, immune modulation through immunosuppression therapy is necessary for successful transplantation of islets without rejection. However, long-term systemic immunosuppression therapy can be toxic to the patient and the islets. Because …
Fret Biosensors: Engineering Fluorescent Proteins As Biological Tools For Studying Parkinson’S Disease, Nathan J. Leroy, Jacob R. Norley, Saranya Radhakrishnan, Mathew Tantama
Fret Biosensors: Engineering Fluorescent Proteins As Biological Tools For Studying Parkinson’S Disease, Nathan J. Leroy, Jacob R. Norley, Saranya Radhakrishnan, Mathew Tantama
The Summer Undergraduate Research Fellowship (SURF) Symposium
Parkinson’s Disease (PD) is a common neurodegenerative disease with over 200,000 new cases each year. In general, the cause of the disease is unknown, but oxidative stress inside of neurons has been associated with the disease’s pathology for some time. Currently, techniques to study the onset of PD inside of neurons are limited. This makes treatments and causes difficult to discover. One solution to this has been fluorescent protein biosensors. In short, these proteins can be engineered to glow when a certain state is achieved inside a cell. The present research discusses the engineering of a genetically-encoded fluorescent protein (FP) …
Multi-Color Ultra-High Resolution Imaging, David A. Miller, Michael Mlodzianoski, Sheng Liu, Fang Huang
Multi-Color Ultra-High Resolution Imaging, David A. Miller, Michael Mlodzianoski, Sheng Liu, Fang Huang
The Summer Undergraduate Research Fellowship (SURF) Symposium
Fluorescence microscopy, which allows multiple-color imaging, plays an important role in observing structures inside cells with high specificity. The advent of super-resolution fluorescence microscopy, or nanoscopy techniques such as single-molecule switching nanoscopy (SMSN), has extended the application range of fluorescence microscopy beyond the diffraction limit, achieving up to 10-fold improvement in spatial resolution. At the same time, the recent development of expansion microscopy (ExM) allows samples to be physically expanded by 4-fold in the lateral dimensions providing another independent method to resolve structures beyond the diffraction limit. When combined, ExM-SMSN makes it possible to achieve another significant leap in resolution …
A Spatial Stochastic Model Of Ampar Trafficking And Subunit Dynamics, Tyler Vandyk, Matthew C. Pharris, Tamara L. Kinzer-Ursem
A Spatial Stochastic Model Of Ampar Trafficking And Subunit Dynamics, Tyler Vandyk, Matthew C. Pharris, Tamara L. Kinzer-Ursem
The Summer Undergraduate Research Fellowship (SURF) Symposium
In excitatory neurons, the ability of a synaptic connection to strengthen or weaken is known as synaptic plasticity and is thought to be the cellular basis for learning and memory. Understanding the mechanism of synaptic plasticity is an important step towards understanding and developing treatment methods for learning and memory disorders. A key molecular process in synaptic plasticity for mammalian glutamatergic neurons is the exocytosis (delivery to the synapse) of AMPA-type glutamate receptors (AMPARs). While the protein signaling pathways responsible for exocytosis have long been investigated with experimental methods, it remains unreasonable to study the system in its full complexity …
Three-Dimensional Microfluidic Tumor Vascular Model For Investigating Breast Cancer Metastasis, Anastasiia Vasiukhina, Brian H. Jun, Luis Solorio, Pavlos P. Vlachos
Three-Dimensional Microfluidic Tumor Vascular Model For Investigating Breast Cancer Metastasis, Anastasiia Vasiukhina, Brian H. Jun, Luis Solorio, Pavlos P. Vlachos
The Summer Undergraduate Research Fellowship (SURF) Symposium
Metastasis is one of the primary reasons for the high mortality rates in female patients diagnosed with breast cancer. It involves the migration of cancer cells into the circulatory system allowing for the dissemination of cancer cells in distal tissues. Understanding the major processes that occur in cells and tissues during metastasis can help improve currently existing therapeutic methods. In order to understand such mechanisms, developing physiologically relevant tissue models is crucial. Advancements in microfluidics have led to the fabrication of 3D culture models with shear stress gradients and flow control that can recapitulate aspects of the tumor microenvironment in …
Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus
Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus
The Summer Undergraduate Research Fellowship (SURF) Symposium
Benzene, a toxin and carcinogen found in air polluted by cigarette smoke, car exhaust, and industrial processes, is associated with the development of leukemia and lymphoma. Other than avoiding exposure, there is no current method to deter the effects of benzene. One potential strategy to prevent these effects is to engineer the bacteria of the human lung microbiome to degrade benzene. To evaluate this novel approach, we must verify that the bacteria remain viable within the lung microenvironment. To do so, lungs were harvested from rats and swabbed to determine the contents of the original lung microbiome. Then green fluorescent …
Mri Applications In Tissue Engineering, Shadi Othman
Mri Applications In Tissue Engineering, Shadi Othman
Science Seminar Series
Shadi Othman of the School of Engineering and Computer Science Bioengineering Program, will speak on his research on MRI applications in tissue engineering.
3d Printing Of Biodegradable Scaffolds For Tissue Engineering Applications, Joe Morin, Michael Pickett, Amy Abraham, Tiera Martinelli
3d Printing Of Biodegradable Scaffolds For Tissue Engineering Applications, Joe Morin, Michael Pickett, Amy Abraham, Tiera Martinelli
The Research and Scholarship Symposium (2013-2019)
With the recent improvements in three dimensional (3D) printing technologies, the potential for tissue engineering and regenerative medicine have significantly improved. One key idea in tissue engineering is to specifically design scaffolds to aid in the healing process by being incorporated into the body’s own tissue. The overall goal of this project is to investigate 3D printable scaffold design to access suitability for tissue replacement. This was accomplished by analyzing the effect of the material used to create the scaffolds, pore size, and pore shape on mechanical stiffness and cell culturability. Based on published literature, it was determined that, depending …
Differentiation Of Kidney Progenitors Using Induced Pluripotent Stem Cells And Conditioned Media Of Renal Cortical Tubular Epithelial Cells, Matthew Nielsen 3644978, Steven Passey, Maxwell Beers, Alonzo Cook
Differentiation Of Kidney Progenitors Using Induced Pluripotent Stem Cells And Conditioned Media Of Renal Cortical Tubular Epithelial Cells, Matthew Nielsen 3644978, Steven Passey, Maxwell Beers, Alonzo Cook
Biomedical Engineering Western Regional Conference
No abstract provided.
Differentiation And Containment Of Derived Pancreatic Beta Cells, Caden Duffy, Alonzo Cook Ph.D.
Differentiation And Containment Of Derived Pancreatic Beta Cells, Caden Duffy, Alonzo Cook Ph.D.
Biomedical Engineering Western Regional Conference
Currently, the only permanent cure for Type 1 Diabetes is a pancreatic or islet transplant. With the shortage of donors, we are progressing research towards alternative therapies by differentiating induced pluripotent stem cells into derived pancreatic β-cells that can be transplanted and used as treatment for Type 1 Diabetes. We are investigating methods to increase the yield of fully derived, insulin producing pancreatic β-cells and are additionally researching the use of hydrophilized expanded polytetrafluoroethylene for use in cell containment devices. These devices could offer a future alternative for islet transplantation in human patients.
Beating Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes On Porcine Extracellular Matrix, Kaitlyn J. Mcentire, Alonzo Cook, Toph Knutson, Gevan Eldredge, Hunter Behrmann, Clayton Holding, Matthew Hodgson, Dillon Despain, Jacob Preslar, Joseph Rich, Matthew Stephens, Matthew Trone, Michael Neff, Sam Worrall, Joshua Mcclellan, Abbie Kondel, Donnie Pfeifer
Beating Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes On Porcine Extracellular Matrix, Kaitlyn J. Mcentire, Alonzo Cook, Toph Knutson, Gevan Eldredge, Hunter Behrmann, Clayton Holding, Matthew Hodgson, Dillon Despain, Jacob Preslar, Joseph Rich, Matthew Stephens, Matthew Trone, Michael Neff, Sam Worrall, Joshua Mcclellan, Abbie Kondel, Donnie Pfeifer
Biomedical Engineering Western Regional Conference
Despite modern technology and developments in heart disease treatment and prevention, heart disease remains the number one cause of death in America. With an inability to meet an ever-increasing demand for heart transplants and the dangers of immunosuppressant drugs, any potential alternative to cardiac transplantation must be pursued. The end goal of this research is to engineer biocompatible tissues that are fully functional to repair or replace damaged portions of the heart following the loss of cardiac function. This research investigates the effect of porcine extracellular matrix as the scaffold for beating IPS-differentiated cardiomyocytes.
Nerve Growth Factor And Lysophosphatidylcholine In Peripheral Nerve Repair, Keaton Karlinsey 8741888
Nerve Growth Factor And Lysophosphatidylcholine In Peripheral Nerve Repair, Keaton Karlinsey 8741888
Biomedical Engineering Western Regional Conference
NGF, Nerve Growth Factor, Lysophosphatidylcholine, Peripheral nerve regeneration, Sciatic nerve, Sciatic crush injury
Cancer Therapeutic Onconase And Lyophilized Cell-Free Protein Expression Systems, Porter Hunt, Bradley C. Bundy, Amin Salehi, Mark Thomas Smith, Jacob Williams, William G. Pitt, Anthony Bennett
Cancer Therapeutic Onconase And Lyophilized Cell-Free Protein Expression Systems, Porter Hunt, Bradley C. Bundy, Amin Salehi, Mark Thomas Smith, Jacob Williams, William G. Pitt, Anthony Bennett
Biomedical Engineering Western Regional Conference
Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis.
Analysis Of Magnetic Resonance Imaging Of Peripheral Nerve Regeneration, Jaron J. Lundwall, Alonzo Cook
Analysis Of Magnetic Resonance Imaging Of Peripheral Nerve Regeneration, Jaron J. Lundwall, Alonzo Cook
Biomedical Engineering Western Regional Conference
Due to accident related neural damage, many people’s lives are impaired or limited in what they can do. Current medical practices are limited at helping distal and proximal nerve stubs regenerate. Many recent research studies have focused on trying to improve this problem by understanding how cut or crushed nerves heal. Our study hopes to help these efforts by improving non-invasive analysis techniques of nerve growth. Magnetic Resonance Imaging (MRI) is one possible solution to creating a reliable analysis technique that in the future could be used on humans. However, current methods of taking MRI scans involve toxic resolving fluid …
Developing A Renal Cell Carcinoma Kidney-Tumor-On-A-Chip To Mimic Tumor-Induced Angiogenesis, Connor Tsuchida, Chris Miller, Ying Zheng
Developing A Renal Cell Carcinoma Kidney-Tumor-On-A-Chip To Mimic Tumor-Induced Angiogenesis, Connor Tsuchida, Chris Miller, Ying Zheng
Biomedical Engineering Western Regional Conference
Currently, models for testing developing cancer therapeutics are inaccurate. To address this issue, the Zheng Lab is developing a kidney-tumor-on-a-chip to mimic tumor-induced angiogenesis. Initial steps were taken toward this ultimate goal by creating an in vitro study between human kidney microvascular endothelial cells and renal cell carcinoma spheroids. The experiments showed an increase in endothelial sprouts in the presence of renal cell carcinoma spheroids vs. normal kidney cortex spheroids.
In Vitro Antioxidant Oxidative Stress Treatment Model In Microgravity, Matt Clegg, Charles P. Harding, Taylor Clegg, Jon Takemoto, Elizabeth Vargis
In Vitro Antioxidant Oxidative Stress Treatment Model In Microgravity, Matt Clegg, Charles P. Harding, Taylor Clegg, Jon Takemoto, Elizabeth Vargis
Biomedical Engineering Western Regional Conference
We propose that the use of individual or a combination of antioxidants (specifically DL-alpha tocopherol phosphate disodium salt, mesobiliverdin, and L-glutathione) with astronauts in microgravity will reduce the effects of oxidative stress caused by ionizing radiation and muscular atrophy.
Molecular Modeling Of Antibody-Antigen Binding Near Solid Surfaces, Derek Bush, Thomas Knotts
Molecular Modeling Of Antibody-Antigen Binding Near Solid Surfaces, Derek Bush, Thomas Knotts
Biomedical Engineering Western Regional Conference
Antibody microarrays are biosensors that have the potential to revolutionize molecular detection in medicine, scientific research, and national defense. However, current microarrays are not widely used due to problems including poor reproducibility and signal quality, unbalanced antibody performance, and cross-reactivity. Prior work in the area focused mainly on the stability of the antibody alone and not its affinity for its antigen. This presentation shows results of using molecular simulation to determine how different types of surfaces affect antigen binding to surface-tethered antibodies. The results offer an unprecedented, molecular-level view into these protein-protein-surface interactions and how to drive binding to occur.