Engineering Commons^™

Bcl2 Mediated Targeted Drug Delivery For The Treatment Of Kidney Fibrosis And Stomach Cancer, Humayra Afrin

Open Access Theses & Dissertations

Apoptosis, the programmed death of cells, is primarily regulated by a delicate balance between pro-apoptotic and anti-apoptotic signals. The Bcl-2 (B-cell lymphoma 2) family of proteins acts as anti-apoptotic agents, promoting cell survival. Dysregulation of these proteins is a common occurrence in conditions such as cancer and fibrosis, where overexpression of anti-apoptotic members can foster tumor cell survival and fibroblast activation. In this study, our aim was to explore the therapeutic potential of Bcl-2 inhibitors, both as a small molecule (specifically Navitoclax (Navi)), inhibitor and as Bcl-2 siRNA, for targeted treatment. Intravenous administration of Navi often leads to thrombocytopenia, necessitating …

Selective Transfection Of A Transferrin Receptor-Expressing Cell Line With Dna-Lipid Nanoparticles And Synthesis Of Parasite-Derived Glycans As Biomarkers For Leishmaniasis, Irodiel Vinales Lozano

Open Access Theses & Dissertations

Despite notable progress in lipid nanoparticle (LNP)-mediated gene delivery, achieving selective transfection of specific cell types, such as cancer cells, remains a significant hurdle, hindering the advancement of innovative gene therapies. In this study, we engineered an LNP formulation encapsulating plasmid DNA (pDNA) encoding the monomeric Green Lantern (mGL) fluorescent reporter protein. The DT7 peptide ligand targeting human transferrin receptor 1 (hTfR1) was also conjugated to the LNP surface for targeted delivery to hTfR1-expressing cells. Optimization of LNP composition yielded favorable particle diameter, ζ-potential, yield, and pDNA encapsulation efficiency. Evaluation of transfection selectivity using a panel of two engineered cell …

Development Of A Cost-Constrained Intelligent Prosthetic Knee With Real-Time Machine Learning, Predictive Stumble Control, Lucas Jonathan Galey

Open Access Theses & Dissertations

The field of biomechatronics is evolving quickly with advances in computer science, biology, and electrical and mechanical engineering. Coupled with increased interests in machine learning (ML) across all industry sectors, there are opportunities to leverage advanced analytics in uniquely complex problems. This study aimed to deploy real-time ML predictions in a novel microprocessor-controlled prosthetic knee (MPK) device capable of identifying and responding to stumble-events to reduce amputee fall prevalence. Innately, stumbling is a chaotic event. Current MPKs operate by detecting gait characteristics and reacting to preprogrammed states. While these systems are beneficial in significant ways, such as energy expenditure and …

Formulation Strategies To Enhance Solubility And Permeability Of Small Molecules For Drug Delivery Applications, Pradeep Kumar Bolla

Open Access Theses & Dissertations

All the new chemical entities/drug molecules intended for therapeutic use must be administered using an appropriate delivery system/dosage form to achieve maximum bioavailability. However, designing a drug delivery system is complex as several factors such as lipophilicity, molecular mass, crystallinity, ionic charge, polymorphic forms and hydrogen bonding) affect the solubility and permeability of these molecules. Biopharmaceutics drug classification system (BCS) categorizes the existing drugs into four classes based on the aqueous solubility and membrane permeability and it is reported that >70% of the drugs are poorly soluble and belong to BCS class II and BCS class IV. Several physical, chemical …

A Cellulose-Based Biological Sensor For The Quantitative Determination Of Glucose And Glycated Hemoglobin (Hba1c), Gilberto Henao-Pabon

Open Access Theses & Dissertations

ABSTRACT

Importance: Diabetes is a common world-wide disease characterized by insulin resistance and as result there is an increased risk of cardiovascular disease and other metabolic abnormalities, that contribute to irreversible end-organ damage. The cause of diabetes is multifactorial and includes genetic, inflammatory and environmental factors plus lifestyle issues. Because in diabetes there are high levels of glucose and glycated hemoglobin, these molecules are used in clinical settings for the acute and chronic management of diabetic patients.

Objectives: a. To design and build an electrochemical biological sensor using the enzyme Glucose Oxidase (GOX) to detect glucose in biological fluids.

b. …

Platelet Biology And Thrombosis: Juul E-Cigarette Exposure And 5ht2a Receptor Drug Discovery, Jean Emmanuel Montes Ramirez

Open Access Theses & Dissertations

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Studies Of Air Pollutants And Their Impact On Respiratory And Cardiovascular Diseases In The El Paso Del Norte Region With Emphasis On Mobile Emissions, Juan Gustavo Arias Ugarte

Open Access Theses & Dissertations

Air pollution may cause different diseases, such as cardiovascular and lung diseases. Traffic is a major source of mobile emissions causing air pollution. Exhaust emissions from mobile sources (Emissions of Carbon Monoxide, Nitrogen Oxides and Hydrocarbons) and Non-exhaust (Brake and Tire) are complex mixtures of gases and dust, including a large number of ultrafine particles, especially in areas with high traffic density. Recent studies have shown high rates of morbidity and mortality in people exposed to these types of emissions because the fine and ultrafine particles can be transported from the respiratory airways to the organs, thus contributing to increase …

Healthcare Utilization Awareness And Labor Monitoring Methodology: A Theoretical I.M.S.E. Approach To Risk Minimization And Human Metrics Capturing In The Nursing Workforce, Luis Reyes

Open Access Theses & Dissertations

The healthcare industry is so complex and dynamic that the development of any tool designed to promote positive patient outcomes nearly requires the developer to be an actual healthcare practitioner. Many healthcare tools exists that initially give the perception that there will be no future requirements that may hinder the tools' integrity, but more often than not, the unexpected occurs. The future is one unpredictable element of life that most engineers wish they could capture with optimal precision. I.M.S.E. (Industrial, Manufacturing and Systems Engineering) applications do not assist with fortune telling, but they do assist with creating robust tools designed …

Optimization Of Collagen Microneedle Using Taguchi Method, Abhilash Aditya

Open Access Theses & Dissertations

Research is conducted to create dissolving collagen microneedles and to optimize the parameters for close to perfect dimension microneedles. A collagen microneedle patch has been developed with a micro-manufacturing process (micro-molding process). The collagen microneedles, varying in length from 300 to 600-μm may reach different targeted layers of the skin depending on the application. As the microneedles penetrate the skin they dissolve, delivering collagen through the epidermis. Such addition of collagen directly to the dermis layers allows it to be absorbed, increasing skin's strength and resilience, since it is one of the natural components of the younger looking skin. This …

Examining The Role Of Attention In Steering Using A Dual Task Paradigm, Venkata Naga Pradeep Ambati

Open Access Theses & Dissertations

Changing the direction of locomotion, often referred to as "steering", is an integral component of human locomotion. Steering requires maintaining dynamic balance while translating and rotating the body in the new travel direction. Given the level of sensation thought to be involved in sub serving goal directed modifications in steering, it is highly likely that steering control may require attention. Since attention resources decline with increasing age, we may see the influence of age on steering performance when attention resources are limited. Therefore, this study sought to investigate the role of attention in steering using a dual task paradigm in …

Organic Photovoltaics: A Charge Transfer Perspective In The Study Of Donor-Acceptor Pairs, Marco Olguin

Open Access Theses & Dissertations

The present research involves the study of donor-acceptor (D/A) dyad complexes from a charge transfer energy perspective. The aim is to provide insight and predictive understanding into the charge transfer processes of the molecular-level components in donor-acceptor based organic solar cells using computational methods to describe photochemical processes at the quantum mechanical level within the Density Functional Theory (DFT) approximation. Predictive understanding is anchored in reproducing experimental results, wherein the present work a perturbative excited-state DFT method is described in detail and shown to give CT energies in excellent agreement with benchmark experimental data. With an accurate excited state method …

Assessment Of Intra-Urban Traffic-Related Air Pollution On Asthmatic Children's Exposure At Schools In The Paso Del Norte Region, Amit Ugamraj Raysoni

Open Access Theses & Dissertations

Traffic-related air pollution can be a major public health concern in any urban area. This problem is compounded in the Paso del Norte region that has experienced rapid economic growth, and a substantial number of people living in close vicinity of major roadways. The desert surroundings, arid weather, frequent temperature inversions, heavy border traffic at the international ports of entry between El Paso and Ciudad Juárez, and poorly maintained vehicle fleet further exacerbates this problem. A growing body of air quality and epidemiologic research has discerned the linkage between traffic emissions and respiratory disorders.

Initial health investigations in the Paso …

Observer-Dependent Model For Analyzing Subjective Parameters In Epidemiology, Milad Zarei

Open Access Theses & Dissertations

Although medical technologies for preventing the contagion and spread of infectious diseases have improved steadily throughout the last century, new infectious diseases are still emerging and spreading swiftly. The modeling of infectious disease spread is crucial in addressing the lack of predictive ability in epidemiology. Managing the spread of infectious diseases requires processing quantitative epidemiological data and the ability to capture the dynamics of the infectious disease in order to provide a measure of control.

In this thesis, I have introducing cognitive biases in diseases spread modeling. For the first time, to the author's knowledge, the human subjective experience has …

Health Prognisis Of Electronics Via Power Profiling, Jonathan Amilcar Cervantes

Open Access Theses & Dissertations

The objective of this research is to investigate a new approach for the early detection of latent defects in electronic devices in the field. Reliability is assessed through the non-traditional approach of recording and evaluating the power profile of electronic devices within a deterministic state of operation. Traditionally, measuring the quiescent current (Iddq) of a device has been employed in manufacturing tests to detect defective parts prior to deployment to the field. However, the monitoring of the deterministic power signature (i.e. boot up or during a self-test routine) has never been exploited to monitor the health of a device in …