Engineering Commons^™

Environment And Response Of 3d-Encapsulated Mesenchymal Stem Cells To Mechanical Loading, Augustus Greenwood

McKelvey School of Engineering Theses & Dissertations

This thesis explores the micromechanical environment induced when cyclically compressing hydrogels via finite element modeling and experimentally on the impact of loading on mesenchymal stem cells (MSCs) when encapsulated withing 3D hydrogel matrices. Degenerative joint diseases, characterized by cartilage degradation, present significant challenges due to cartilage's limited self-repair capacity. Innovative approaches, including stem cell-based therapies and engineered biomaterials, have emerged as promising strategies for cartilage repair and regeneration. This work specifically investigates the calibration of a bioreactor, the uniformity of load response across the hydrogel constructs via finite element modeling (FEM), and the stress response of MSCs subjected to various …

Enhanced Fabrication Of Microdroplet Generator Nozzle Arrays: Optimizing Koh Etching For Microfluidic Applications, Hongyu Bai

McKelvey School of Engineering Theses & Dissertations

Ultrasonic microdroplet generators are useful devices with broad applications ranging from aerosolized drug delivery to three-dimensional (3D) printing-based additive manufacturing. One such technology comprises a microfabricated array of nozzles with droplet production driven by a piezoelectric transducer. The present study focuses on refining a critical fabrication step, anisotropic wet etching of pyramidal nozzles using a basic potassium hydroxide (KOH) solution. Given the integral role of nozzle geometry in device operation, high-precision techniques including Reactive Ion Etching (RIE), Deep Reactive Ion Etching (DRIE), and KOH wet etching were employed. A tapering geometry is preferred for acoustic wave focusing and efficient droplet …

An Apparatus To Quantify Lengthwise Flexural Rigidity Profiles Of Endovascular Devices, Charles B. Suskin

McKelvey School of Engineering Theses & Dissertations

Endovascular procedures require access to distal anatomical sites through the vasculature using catheters and guidewires. Quantitative frameworks for device behavior during procedures hold the potential to drive device design through greater understanding of the mechanical behavior of endovascular devices, and offer the potential to personalize care based on a patient's particular vascular anatomy. However, data that would facilitate this technology are lacking, partly due to undisclosed material properties from manufacturers and partly due to the intricate variations along the length of each device due to material changes and the intersections between them. We developed a three-point bend test methodology on …

Calculating The Difference In Stiffness Of Living T Cells Through Micropipette Aspiration, Minju Lee

McKelvey School of Engineering Theses & Dissertations

Cardiovascular disease (CVD) accounted for 17.9 million deaths in 2019, with fibrosis contributing to nearly a quarter of these fatalities [1,2]. Fibrosis, characterized by excessive connective tissue formation, has been strongly linked to T cells, essential components of the immune system. This study explores the mechanisms of T cell activation and the subsequent changes in biophysical properties like diameter, stiffness, and elasticity, aiming to develop therapeutic strategies for fibrosis-related diseases, including CVD. Utilizing the micropipette aspiration technique, we accurately assessed T cell stiffness and observed a change in bulk cell stiffness upon activation. The results demonstrated increased fluid-like behavior in …

Performance And Emissions Study Of N+3 And N+4 Engine Models With Several Fuel Types Using Npss, Abel Solomon

McKelvey School of Engineering Theses & Dissertations

The aviation industry is known to be one of the major contributors to greenhouse gases accounting for 4.9% of the global greenhouse emissions. With the ever-increasing threat of climate change to the overall survival of the planet, the exploration of new technologies and alternative energy sources that minimize greenhouse gas emissions are of paramount importance. In this regard, the development of propulsion systems well suited for the performance and emissions requirements of future commercial aircraft plays a crucial role. This thesis investigates N+3 and N+4 technology-level propulsion systems that are proposed by NASA as possible propulsion systems for advanced single-aisle …

Engineered Material Systems For Mimicking Tissue And Disease, Margrethe Ruding

McKelvey School of Engineering Theses & Dissertations

This thesis comprises two studies involving design and application of soft material systems. The goal of the first study was to design, fabricate, and characterize hydrogel lattice structures with consistent, controllable, anisotropic mechanical properties. Lattices, based on four types of unit cells (cubic, diamond, vintile, and Weaire-Phelan), were printed using stereolithography (SLA) of polyethylene glycol diacrylate (PEGDA). In order to create structural anisotropy in the lattices, unit cell design files were scaled in one direction by a factor of two in each layer and then printed. The mechanical properties of the scaled lattices were measured in shear and compression and …

Three-Component Composite Phase-Change Material For Thermal Regulation, Bidisha Ojha

McKelvey School of Engineering Theses & Dissertations

Phase change materials can increase the efficiency of many energy-intensive applications, such as solar power plants, solar heating and cooling systems, heat recovery systems, photovoltaic electricity systems, and Earth satellite systems. In several proposed thermal management systems for high power electronic equipment, PCMs change phase and absorb latent heat at peak energy loads during operation, then dissipate this energy later to prevent overheating. More specifically, composite PCM’s, consisting of an organic material (e.g., paraffin) and an inorganic material (e.g., metallic alloys or salt hydrates), provide a superior balance of thermal conductivity and latent heat for thermal management. Organic PCMs have …

Modeling, Analysis, And Simulation To Reveal The Mechanisms Of Ciliary Beating, Louis Woodhams

McKelvey School of Engineering Theses & Dissertations

Cilia are microscopic cellular appendages that help us breathe by clearing our airways, maintain the health of our central nervous system by circulating cerebrospinal fluid, and allow us to reproduce by transporting eggs and propelling sperm cells. Cilia even determine the asymmetry of our internal organs during embryonic development. However, the mechanisms underlying ciliary beating are not fully understood. Questions remain as to how arrays of the motor protein dynein generate the propulsive waveforms observed in cilia and how structural elements within the cilium and its connection to the cell deform during beating. In the current work, mathematical modeling, analysis, …

Droplet Dynamics And Heat Transfer During Condensation On Lubricant-Infused Surfaces, Jianxing Sun

McKelvey School of Engineering Theses & Dissertations

Inspired by pitcher plants, a micro/nano- structured solid surface infused with low-surface-tension lubricant constitutes a novel class of biomimetic surfaces—lubricant-infused surfaces (LISs). Unlike solid hydrophobic and superhydrophobic surfaces, a LIS is “hemi-solid and hemi-liquid”. The thin lubricating film can extraordinarily decrease adhesion and avoid contact line pinning between the working fluid and the underlying solid, displaying many desirable properties, such as excellent liquid repellency, suppression of fouling, and self-healing. Hence, LISs are ideal for applications where liquids or contaminants must be efficiently removed from surfaces in harsh environments. For example, LISs can promote stable dropwise condensation of water or low-surface-tension …

A Modification To The Skiving Process For The Manufacture Of Offset Geometry Strip-Fin Heat Sinks, Daniel Luberda

McKelvey School of Engineering Theses & Dissertations

High-powered electronics are extremely prevalent, and a vast majority of these devices use some form of heat sinks as either an active or passive cooling device. Although two-phase cooling technology has rapidly advanced to accommodate increasing heat loads on modern electronics, the added costs and complication inherent in two-phase cooling solutions makes effective air-cooled heat sinks potentially valuable.

A more effective heat sink can occupy a smaller footprint to dissipate the same heat flux, meaning material and packaging costs can be reduced. Aluminum heat sinks are produced through a variety of manufacturing processes, including machining, bonding, extrusion, casting, forging, 3D …

Foundations For Finite-State Modelling Of A Two-Dimensional Airfoil That Reverses Direction, Jake Michael Oscar Welsh

McKelvey School of Engineering Theses & Dissertations

Current 3-D finite-state wake models are incapable of simulating a maneuver in which the sign of the free-stream velocity changes direction and the rotor enters its own wake -- as might occur in the case of a helicopter which ascends and then descends. It is the purpose of this work to create a 2-D finite-state wake model which is capable of handling changes in free-stream direction as a precursor to development of a 3-D model that can do the same.

The 2-D finite-state model used for reentry modifications is an existing model created by Peters, Johnson, and Karunamoorthy. By the …

Particle Formation And Thermal Radiation In Laminar Diffusion Flames With Applications To Energy And Materials, Phillip Irace

McKelvey School of Engineering Theses & Dissertations

Fossil fuels supply over 80% of the world’s primary energy, and if current policy and technology trends continue, global energy and energy-related carbon dioxide (CO2) emissions are predicted to increase for at least several decades owing to population and economic growth, leading to serious concerns regarding global warming. Natural gas releases less CO2 than other fossil fuels (e.g., coal and oil) and can help meet future CO2 emission targets. Natural gas combustion, however, has poor radiation heat transfer when compared to other fossil fuels owing to its low propensity for soot formation, making it difficult to use as a drop-in …

Development And Application Of A Novel Acoustic Microfluidic Technology For Single Cell Per Well Trapping And High-Resolution Analysis Of Cilia Motion In Chlamydomonas Reinhardtii, Mingyang Cui

McKelvey School of Engineering Theses & Dissertations

Acoustic manipulation of cells and microorganisms is a label-free and contact-free technique with promise for biological and biomedical applications. When exposed to an ultrasonic standing wave field, particles suspended in microfluidic channels will be moved to pressure minima (nodes) or maxima (antinodes) due to the acoustic impedance mismatch between particles and the suspension medium. Cilia motion is fundamental to understanding biological and biomedical problems related to dysfunctional human cilia, including primary ciliary dyskinesia, blindness, and male infertility. However, in vivo and ex vivo mammalian ciliated cell research is laborious and time-consuming due to difficulty in growing, maintaining, and imaging these …

Defining The Role Of Elastic Fibers In Tendon Mechanics, Jeremy D. Eekhoff

McKelvey School of Engineering Theses & Dissertations

Tendons serve as a linking component of the musculoskeletal system by transferring forces between muscle and bone. As such, the structural proteins of the tendon extracellular matrix are of vital importance for the tissue to function properly and maintain its mechanical integrity. Collagen is the principal constituent of tendon and makes up its aligned hierarchical organization. Other structural proteins, such as elastin, are in comparison understudied and not well understood in relation to tendon function. Elastin, the main component of elastic fibers, has unique mechanical properties including high extensibility, fatigue resistance, and elasticity; these properties are important for elastin-rich tissues …

Acoustic Radiation Force And Its Application For Cell Manipulation And Ion Channels Activation, Xiangjun Peng

McKelvey School of Engineering Theses & Dissertations

Sound is a stress wave that carries energy and momentum flux. Scattered sound waves can generate acoustic radiation force that can be used to manipulate particles or cells. This dissertation demonstrates the physics behind cell manipulation by ultrasound. The work begins with a detailed analysis of the mechanics of using standing surface acoustic waves to fabricate acoustic tweezers for contactless particle manipulation using acoustic radiation force. Models to design and analyze acoustic radiation force have traditionally relied on plane wave theories that cannot predict how standing surface acoustic waves can levitate cells in the direction perpendicular to the substrate. We …

Attachment Of Fibrous Materials In Nature And Surgical Repair, Ethan Daniel Hoppe

McKelvey School of Engineering Theses & Dissertations

Mother nature is the ultimate problem solver. Often by brute force and necessity for survival, the organism that has the best solution to a problem is the one to proliferate. One of these problems for which nature provides an elegant solution is the attachment of dissimilar materials. This dissertation explores the strengthening and relaxation of interactions between soft and hard materials, typically with one of the two being fibrous in character, with the goal of identifying strategies for improving the surgical reattachment of tendon to bone. The work begins with a study of the plant Harpagonella palmeri, which the dissertation …

Investigation Of Microdroplet Generation, Morphological Evolution, And Applications Under Quasi-Steady And Dynamic States, Li Shan

McKelvey School of Engineering Theses & Dissertations

Microscale droplets are commonly encountered in the fields of materials processing, thermal fluids, and biology. While these droplets are naturally occurring, recent advances in microfabrication have enabled researchers to harness their enhanced transport characteristics for numerous laboratory and industrial applications from controlled chemical synthesis to inkjet printing and thermal management. Smaller droplets have larger specific surface area and a greater perimeter-to-area ratio when resting on a surface (i.e., sessile), which accelerates processes occurring at droplet surfaces like evaporation, chemical reaction, or combustion. The demand for microdroplets with smaller and more uniform sizes has motivated investigation of how such droplets can …

Numerical Simulation Of Flow Past An Airfoil With Ice Accretion On Leading Edge, Boyu Wang

McKelvey School of Engineering Theses & Dissertations

The focus of this research is on aerodynamic simulation of flow past NACA 23012 airfoil with clean surface and with ice accretion on its leading edge by using the commercial CFD solver ANSYS Fluent. Reynolds-Averaged Navier-Stokes (RANS) computations are performed using Spalart-Allmaras (SA) and Wray-Agarwal (WA) turbulence models. ANSYS mesh package ICEM is used to model the geometry and generate the mesh. The computations are performed at 0, 2, 4, 6, 8, 10, and 12 degrees angle of attack which are compared with experimental data. For the case of ice accretion at the leading edge, the physical geometry becomes more …

Mechanics Of Catheter Herniation, Chase Hartquist

McKelvey School of Engineering Theses & Dissertations

Endovascular catheter-based technologies have revolutionized the treatment of complex vascular pathology. Catheters and endovascular devices that can be maneuvered through tortuous arterial anatomy have enabled minimally invasive treatment in the peripheral arterial system. Although mechanical factors drive an interventionalist's choice of catheters and sheaths, these decisions are mostly qualitative and based on personal experience and procedural pattern recognition. The field currently lacks a definitive quantitative characterization of endovascular tools that are best suited for specific peripheral arterial beds. This approach gives rise to a phenomenon called catheter herniation, which occurs when a catheter is energetically displaced or buckles while a …

Computational Fluid Dynamics And Fluid Structure Interaction Research On Flow In A Glenn Shunt And A Flexible Tube And On A Cantilevered Plate, Chunhui Wang

McKelvey School of Engineering Theses & Dissertations

This thesis employs Computational Fluid Dynamics (CFD) simulation technology to solve three flow problems: (1) Blood flow in a Bidirectional Glenn Shunt and a combined Bidirectional Glenn Shunt (BGS) and Blalock-Taussing (BT) Shunt. This shunt is used to address the problem of Cyanosis or “Blue Baby Syndrome,” which is an infant disorder that affects the newly born babies whose skins turn blue or purple because of lack of necessary blood flow between heart and lung due to pulmonary vascular blockage. The goal of this study was to evaluate the performance of BGS and combined BGS+BT shunt in achieving the desired …

Post-Traumatic Elbow Contracture Characterization And Physical Therapy-Based Treatment Strategies In A Preclinical Model, Alex Reiter

McKelvey School of Engineering Theses & Dissertations

The elbow is the most commonly dislocated joint in the pediatric population and second most common in adults. As one of the most congruous joints in the body, slight changes in biomechanics due to injury can lead to drastic reductions in range of motion causing potential quality of life issues. Post-traumatic joint contracture occurs in 12% of patients following elbow dislocation or fracture, and it is characterized by a loss in ROM, joint stiffness, and pain. Preventing joint contracture and functional deficits from occurring is one of the primary goals when managing these injuries. A rat model of joint contracture …

Understanding The Interactions Between Acoustic Fields And Motile Microorganisms In Microfluidic Systems, Minji Kim

McKelvey School of Engineering Theses & Dissertations

Acoustofluidics utilizes ultrasonic standing waves in microscale fluidic channels to manipulate cells, microorganisms, and other objects sized from tens of nanometers to tens of microns. When exposed to an ultrasonic standing wave field, particles suspended in a fluid become confined to potential minima (nodes) of the acoustic field. I will present a number of related studies that involve the interactions between acoustic fields and motile microorganisms. First, I will show how an acoustic trap-and-release method enables rapid quantification of cell motility. As a demonstration, the newly developed motility assay is applied to discriminate swimming of wild-type and mutant Chlamydomonas reinhardtii …

Subject-Specific Musculoskeletal Modeling Of Hip Dysplasia Biomechanics, Ke Song

McKelvey School of Engineering Theses & Dissertations

Developmental dysplasia of the hip (DDH) is characterized by abnormal bony anatomy, causes pain and functional limitations, and is a prominent risk factor for premature hip osteoarthritis. Although the pathology of DDH is believed to be mechanically-induced, little is known about how DDH anatomy alters hip biomechanics during activities of daily living, partly due to the difficulties with measuring hip muscle and joint forces. Musculoskeletal models (MSMs) are useful for dynamic simulations of joint mechanics, but the reliability of MSMs for DDH research is limited by an accurate model representation of the unique hip anatomy. To address such challenges, this …

Analysis Of Fluid Flow In Redox Flow Batteries, Erfan Asadipour

McKelvey School of Engineering Theses & Dissertations

Redox flow batteries (RFB) hold great potential for large-scale stationary energy storage. However, their low energy density compared to other energy storage systems must improve for feasibility. Electrolyte flow distribution affects current density distribution and providing a uniform current density distribution is one way to improve RFB performance. Additionally, reducing the power consumption of the electrolytes’ pump as a source of energy loss in RFB systems increases their efficiency. Investigating both subjects requires analysis of the fluid dynamics in RFB cells.

In this thesis, a novel, computationally cost-effective hydraulic-electrical analogous model (HEAM) was developed to study fluid dynamics by implementing …

Design And Optimization Of An Array Of Hollow Micropillar Structures For Enhanced Evaporative Cooling, Zhikai Yang

McKelvey School of Engineering Theses & Dissertations

With the increasing demand for higher-performance chips, the architecture of these semiconductor devices becomes more complex, leading to the need for higher-performance cooling technologies. For example, 3D stacked chips offer several advantages, including mixed functionality, reduced signal delay, and a smaller footprint. However, these devices yield higher heat densities due to the heat compounded from one stacked die to the next. While traditional single-phase cooling technologies can dissipate large heat fluxes, its performance is inversely proportional to its hydraulic diameter leading to larger required pumping powers. Two-phase cooling is a promising technique for dissipating high heat fluxes by utilizing the …

Mechanical Strength Of Germanium Doped Low Oxygen Concentration Czochralski Silicon And The Effect Of Oxygen On Nitrogen Dissociation In Silicon, Junnan Wu

McKelvey School of Engineering Theses & Dissertations

During the Czochralski growth of silicon, it is inevitable for oxygen to be incorporated into the silicon crystal from the quartz crucible. Interstitial oxygen improves the mechanical strength of silicon by pinning and locking dislocations, but also generates thermal donors during device processes, shifting the electrical resistivity. For silicon wafers used in radio frequency (RF) applications, it is important to ensure the high resistivity of the substrates for good RF characteristics. Therefore, the oxygen level in these high resistivity silicon wafers is kept very low (< 2.5 × 1017 atoms/cm3) by carefully controlling the Czochralski growth conditions, in order to reduce the thermal donor concentration to an acceptable level. Silicon on insulator (SOI) substrates made from high resistivity wafers have been widely used for RF applications. SOI manufacturing includes multiple high temperature thermal cycles (1000 – 1100 °C), during which the high resistivity wafers are prone to slip and warpage. Therefore, it is technologically important to recover some of the lost mechanical strength due to the lack of oxygen by introducing electrically inactive impurities to suppress the dislocation generation and mobility in silicon. Germanium (Ge) as an isovalent impurity is 4% larger in size and forms a solid solution with silicon in the entire concentration range. Previous works have shown Ge doping at high concentrations above 6 × 1019 atoms/cm3 increased mechanical strength of silicon with high oxygen concentration (~ 1 × 1018 atoms/cm3). In this work, we explore the effect of Ge doping (7 - 9 × 1019 atoms/cm3) on the mechanical strength of low oxygen concentration (< 2 × 1017 atoms/cm3) silicon, where the oxygen associated dislocation locking and pinning are very low. A mechanical bending test was used to study the average dislocation migration velocity and the critical shear stress of dislocations motion at 600 – 750 °C for Ge doped, nitrogen doped, and undoped low oxygen samples, as well as nitrogen doped float-zone and un-doped high oxygen concentration samples. Next, we fabricated SOI substrates using these high resistivity wafers and compared their slip generation rates and the slip-free epitaxial grow temperature windows after the high temperature thermal cycles (> 1000 °C). Our results indicate at lower temperature Ge doesn’t affect the dislocation mobility …

New Modeling Approaches For The Prediction Of Combustion Pollutants, Phillip R. Johnson

McKelvey School of Engineering Theses & Dissertations

Combustion processes are ubiquitous to human technological development and provide many benefits such as large-scale power generation for electricity and transportation along with residential and commercial heating for manufacturing, cooking, and warmth. However, these various processes can also have harmful effects on human health and the environment via emission of CO2 and other pollutants such as NOx and particulate matter (PM; often in the form of soot). For these reasons, there is a continued need for controlling, improving, and optimizing combustion processes. Modeling of these processes provides powerful insights into system-level dynamics and their control. Due to the size and …

Aging Effects On Arterial Mechanics And Matrix Remodeling, Jie Hawes

McKelvey School of Engineering Theses & Dissertations

Large elastic arteries are a composite structure composed of cells and extracellular matrix proteins. Passive arterial mechanical behavior is determined by the composition of extracellular matrix proteins, in particular elastin and collagen. Elastin provides reversible elasticity to the large elastic arteries during cyclic loading and dampens the pulsatile flow from the left ventricle, reducing the workload on the heart and protecting the end organs. Disorganization and insufficiency of elastin alters the passive mechanical behavior of the large arteries. The arterial wall responds to changes in elastin organization or amount through matrix remodeling. Aging causes elastin fragmentation and degradation which changes …

Blood Flow Simulation Of Particle Trapping In Models Of Arterial Bifurcations, Qihang Xu

McKelvey School of Engineering Theses & Dissertations

This thesis describes the particle trapping mechanism in blood flow in different arterial bifurcation models. For validation of CFD calculations, a T-junction model and a Y-junction model are analyzed. In both the models, there is one inlet pipe with two outlet pipes creating a symmetric bifurcation at some angle from the centerline of the inlet pipe. Naiver-Stokes (RANS) equations are solved for single phase laminar flow using the commercial CFD software ANSYS Fluent. After validation, Eulerian simulations are performed by using the Discrete Phase Model (DPM) for two-phase flow with particles injected in different bifurcation models with bifurcation angle of …

Exploring Attacks And Defenses In Additive Manufacturing Processes: Implications In Cyber-Physical Security, Nicholas Deily

McKelvey School of Engineering Theses & Dissertations

Many industries are rapidly adopting additive manufacturing (AM) because of the added versatility this technology offers over traditional manufacturing techniques. But with AM, there comes a unique set of security challenges that must be addressed. In particular, the issue of part verification is critically important given the growing reliance of safety-critical systems on 3D printed parts. In this thesis, the current state of part verification technologies will be examined in the con- text of AM-specific geometric-modification attacks, and an automated tool for 3D printed part verification will be presented. This work will cover: 1) the impacts of malicious attacks on …