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Cryopreservation Of Adipose Derived Adult Stem Cells And Multidimensional Cell Sheets, Sreedhar Thirumala
Cryopreservation Of Adipose Derived Adult Stem Cells And Multidimensional Cell Sheets, Sreedhar Thirumala
LSU Doctoral Dissertations
Human adipose tissue provides a uniquely abundant and accessible source of adult stem cells. In response to chemical, hormonal or structural stimuli, these human adipose-derived adult stem (ASCs) cells can differentiate along multiple lineage pathways, including adipocytes, chondrocytes, myocytes, neurons and osteoblasts. Successful cryopreservation of scientifically and commercially important ASCs would revolutionize the tissue engineering and regenerative medicine industry. In this research, we have investigated the water transport phenomenon during freezing of several passages of adipose derived adult stem cells and generated the membrane permeability parameters in the presence and absence of cryoprotective agents. These measured permeability parameters were then …