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Protein-Specific Empirical Model Of Protein Adsorption On Surfaces, Dan Nicolau

Single-use Technologies II: Bridging Polymer Science to Biotechnology Applications

Predicting protein adsorption from solution to a surface is an important problem in biomedicine and related fields. Despite constant attention in the literature, it is not currently possible to predict quantitatively the amount of adsorbed protein given environment, protein and surface parameters. We present a purely empirical approach to predict protein adsorption using a linearly piecewise model with breakpoint, based on progress in the understanding and quantification of physico-chemical properties on protein molecular surfaces. This model is capable of accounting for over 90% of the variance in the data, despite the fact that the adsorption data spans over three orders …