Engineering Commons^™

Application Of Metagenomic And Molecular Microbiology Techniques To Elucidate Sources Of Fecal Pollution And Anthropogenic Antibiotic Resistance Genes To Surface Water: A Step Towards A “One Health” Approach To Watershed Management, Mehedi Hasan Tarek

Graduate Theses, Dissertations, and Problem Reports

The use of fecal indicator bacteria, such as Escherichia coli, is a widely established regulatory and monitoring practice to detect surface water contamination associated with fecal pollution. However, the detection or quantification of fecal indicator bacteria alone does not accurately inform the sources of fecal pollution. The development of molecular and metagenomic methods that target the DNA of microorganisms has resulted in a host of new tools for monitoring fecal pollution and its sources, as well as for understanding emerging microbial threats, such as antimicrobial resistance. Antimicrobial resistance is a critical “One Health” challenge presenting a high risk to …

Optimizing Sample Collection And Data Interpretation For Effective Wastewater-Based Epidemiology In Combined Sewer Systems, Christopher Allen Anderson

Graduate Theses, Dissertations, and Problem Reports

COVID-19 has spurred growth in the science surrounding wastewater-based epidemiology (WBE) pertaining to the detection of severe acute respiratory virus 2 (SARS-CoV-2) in waste streams as an early warning signal for public health. However, the highly variable wastewater environment has made it difficult to standardize an approach for sampling and analysis, especially in locations using combined sewer infrastructure. This study addresses knowledge gaps of WBE via three specific aims: (1) to compare diurnal fluctuations of SARS-CoV-2 and the human fecal indicator, pepper mild mottle virus (PMMoV) in wastewater treatment plant (WWTP) influent samples collected during dry versus wet weather conditions; …

Concentrated Wastewater Treatment Using A Ferric Iron-Dosed Anaerobic Upflow Sludge Blanket Reactor For Recovery Of Phosphorus And Ammonium, Carley E. Shingleton

Graduate Theses, Dissertations, and Problem Reports

This study evaluated the performance of a novel, ferric iron-dosed anaerobic bioreactor to recover two separate nutrient products from concentrated wastewaters -- phosphorus as vivianite (Fe₃(PO₄)₂×8H₂O) and an ammonium-containing effluent with low organics. A bench-scale upflow anaerobic sludge blanket (UASB) reactor fed with a synthetic wastewater (458.0 mg/L total organic carbon, 282.7 mg/L ammonium, and 84.4 mg/L phosphate) was dosed with a ferric chloride solution at an org. C/Fe³⁺ molar ratio ~17.5 to facilitate organic carbon oxidation coupled to iron reduction. The reactor design allowed natural settling of vivianite to its …

Spatiotemporal Trends In Particle-Associated Microbial Communities In A Chlorinated Drinking Water Distribution System, Madison Haddix

Graduate Theses, Dissertations, and Problem Reports

Bacteria can survive treatment and enter drinking water distribution systems (DWDSs), or enter DWDSs through intrusion or maintenance work on the system. DWDSs can also be suitable environments for bacterial regrowth. Many factors can affect the microbial community composition of DWDSs, including various spatiotemporal, hydraulic, and water quality parameters. Although most bacteria within DWDSs are harmless, opportunistic pathogens, such as Legionella pneumophila, Pseudomonas aeruginosa, and Mycobacterium avium, can inhabit DWDSs and premise plumbing and are the leading cause of waterborne disease outbreaks in developed countries. Previous studies have attempted to establish relationships between various parameters and microbial …

Development Of A Framework To Support Community-Scale Nutrient Recovery For Local Crop Fertilization And Production, Scott A. Lopez

Graduate Theses, Dissertations, and Problem Reports

Nutrient and resource recovery (NRR) has become increasingly crucial as regions face deteriorating sanitation infrastructures, limited support, and growing environmental challenges. Implementing region-specific NRR technologies and systems could provide effective circular economy insights into addressing these challenges. Because of the multidisciplinary challenges associated with the design and implementation of NRR strategies, various government and local decision-makers need to collaborate effectively in the decision-making process. Structured decision-making (SDM) methodologies are practical when determining the most appropriate NRR strategy for nutrient-rich waste streams. However, applications of computational SDM limited because of the vast amounts of data that are needed to analyze the …

Development Of An Innovative Co-Treatment Technology For Produced Water And Blowdown Water: A Regional Approach Of Water Management For Energy Production, Golnoosh Khajouei

Graduate Theses, Dissertations, and Problem Reports

Freshwater use for power generation represents the second-largest water use globally. In the United States, freshwater withdrawals for thermoelectric power accounted for 38% of the total freshwater withdrawals for all uses in 2010. Cooling systems are the most water-intensive part of the thermoelectric generation process. For instance, one 300 MW power generator required about 20,000 m³/h circulating cooling water. The cooling operation discharges a large volume of wastewater in the form of blowdown water (10-20% of the consumed water) that requires treatment for reuse or surface discharge. Produced water (PW), the fluid which returns to the surface from …

Climatic And Land Cover Sensitivities Of Stormwater Runoff In A Coastal Natural-Urban Basin Of Texas, Usa., Nusrat Nasrin Khan

Graduate Theses, Dissertations, and Problem Reports

Changes in climatic and land use drivers typically drive stormwater runoff quantity and quality. This study focuses on determining the stormwater runoff sensitivities to variation in climatic and land cover changes in a coastal natural-urban basin. A mechanistic hydrologic model was developed for the Spring San Jacinto Basin (SSJB) of Texas, USA considering the basin as a pilot study area by using the U.S. Environmental Protection Agency (EPA)’s Storm Water Management Model 5.2. The SSJB drains an area of 1949 km² ultimately into the northern Gulf of Mexico, USA through peripheral neighborhoods of the Greater Houston area. The model …

Application Of Artificial Intelligence For Co2 Storage In Saline Aquifer (Smart Proxy For Snap-Shot In Time), Marwan Mohammed Alnuaimi

Graduate Theses, Dissertations, and Problem Reports

In recent years, artificial intelligence (AI) and machine learning (ML) technology have grown in popularity. Smart Proxy Models (SPM) are AI/ML based data-driven models which have proven to be quite crucial in petroleum engineering domain with abundant data, or operations in which large surface/ subsurface volume of data is generated. Climate change mitigation is one application of such technology to simulate and monitor CO2 injection into underground formations.

The goal of the SPM developed in this study is to replicate the results (in terms of pressure and saturation outputs) of the numerical reservoir simulation model (CMG) for CO2 injection into …

Predictions Of Produced Water Quality And Recycled Water Optimization For Spatially-Distributed Wells In Point Pleasant Formation, Armel Quentin Mbakop

Graduate Theses, Dissertations, and Problem Reports

The treatment of produced water as a fracturing fluid is becoming an increasingly important aspect of water management surrounding the booming of the unconventional oil and gas industry. Two main problems facing the oil and gas industry are the availability of water for well drilling and completion and disposal of the produced water. Unconventional well drilling and completion in the Utica shale requires large amounts of water. The wastewater that results after production—containing high levels of organic and inorganic matter— is usually disposed of through deep well injection. A new approach reuses this produced water as part of subsequent fracturing …

Stormwater Runoff Response Under Changing Climate And Land Uses Across Gradients Of Inland And Coastal Urban-Natural Basin, Mahmood Khan

Graduate Theses, Dissertations, and Problem Reports

Climatic variability has caused significant shifts in the magnitude, frequency and spatiotemporal distribution of precipitation, generating excessive runoff that leads to frequent occurrences of extreme hydrologic events such as the pluvial (rainfall accumulation) and fluvial (riverine) floods. The conversion of natural areas into urban lands is further exacerbating the rainfall accumulation by increasing surface imperviousness that hinders infiltration of water into the soil. This dissertation predicts and characterizes potential shifts in the future annual stormwater runoff
volumes and runoff extremes under the standalone and coupled changes in climate and land use across inland and coastal urban-natural settings. The research was …

Detection Of Fecal Bacteria In Watersheds Impacted By Acid Mine Drainage, Claire Addison Mcdonald

Graduate Theses, Dissertations, and Problem Reports

Escherichia coli, a fecal coliform, is an essential component of water quality monitoring, entering waterways through sewage deposits, agricultural land and livestock, and wildlife. Various fast and user-friendly methods for quantifying E. coli make it the principal indicator bacteria for fecal contamination. The most common methods for E. coli quantification are culture-based and involve cultivating samples onto nutrient agar and counting the visible colonies. However, E. coli can enter a viable but non-culturable (VBNC) state in stressful environments. Bacteria in a VBNC state are still alive but reduce key metabolic activities that typically encourage growth and division, allowing them …

Evaluating Potential Soil Amendments And Geomorphic Methods For Mine Land Reclamation, Amanda Rodrigues Silva

Graduate Theses, Dissertations, and Problem Reports

The United States has approximately 22% of the world’s coal reserves. Since 1961, the electric power sector has been the major user of coal in the U.S., accounting for 91.9% of the coal consumed in the country in 2021. Sixty-two percent of total U.S. coal production in 2021 was sourced from surface mining, and Wyoming, West Virginia, Pennsylvania, Illinois, and Montana were responsible for 74% of this total production.

Coal-mining activities expose sulfide minerals in rocks that, when in contact with oxidizing conditions, produce sulfate-rich drainage known as acid mine drainage (AMD). AMD treatment typically involves chemical treatments to raise …

Stormwater Sensitivities To The Climatic And Land Cover Drivers In A Heavily Urbanized Coastal Basin Along The Northern Gulf Of Mexico, Usa., Md Faisal Razy

Graduate Theses, Dissertations, and Problem Reports

Stormwater runoff response in a complex coastal-urban basin varies with the changes in climatic and land cover drivers. This thesis aims to assess the runoff responses to variation in climatic and land cover variables and perform a sensitivity analysis in complex coastal-urban basins. The Buffalo San Jacinto Basin (BUSJ) of southeast Texas, USA was considered a pilot study area for this research. BUSJ is a heavily urbanized coastal basin, draining an area of 3132 km² through the City of Houston, Texas into the northern Gulf of Mexico, USA. A process-based hydrological model was developed with fine spatio-temporal resolutions for …

Elucidating Biogeochemical Reactions Of Iron For Ammonium Removal And Nutrient Recovery From Wastewaters: Moving Toward Sustainable Wastewater Management, Rifat Anwar

Graduate Theses, Dissertations, and Problem Reports

Development and applications of anaerobic treatment technologies has been discussed as an effective alternative to conventional aerobic processes for removal of nitrogen from wastewaters. With abundance of iron and its high reduction potential, employing ferric reduction-based reaction pathways in engineering processes could be an effective strategy to promote energy efficiency of wastewater treatment. In particular, ferric reduction coupled to ammonium oxidation (Feammox), a novel microbial metabolic function recently found to occur under anaerobic/anoxic condition through iron dosing, can be used for nitrogen pollutant removal. Iron dosing for wastewater treatment could also promote other microbial and chemical reactions that facilitate nutrient …

Evaluating Effects Of Sediment On Microbial Loading In A Drinking Water Distribution System, Erika Osborne

Graduate Theses, Dissertations, and Problem Reports

Drinking water distribution systems (DWDS) are critical for protecting public health by ensuring safe water is transported from centralized treatment facilities to consumers. Sediment accumulation in DWDS is a common challenge that water utilities face that may pose some health risks when waterborne pathogens colonize biofilms formed on the sediment surface. Water utilities often establish flushing routines to help mitigate sediment accumulation in their system but the role that sediment plays in contributing to the total microbial loading in the system is unclear. The main objective of this study was to determine if sediment in a DWDS presents a significant …

Effects Of Changes In Land Use Land Cover And Climate On Long-Term Total Dissolved Solids Trends In The Mid-Atlantic Region Of The United States, Melaine Edouard Kolimedje

Graduate Theses, Dissertations, and Problem Reports

The Mid-Atlantic region (MAR) of the U.S. is subjected to a variety of stressors that affect the headwaters of the major rivers. Some of these stressors are abandoned mine drainage, agriculture, municipal point sources, urban areas, out-of-basin diversions, competing water uses, rapid population growths in the lowlands, alterations in water availability due to climate change and habitat alteration. In addition to these regional stressors, the rapid population growths and energy sources shifting have resulted in changes in land use and land cover (LULC) over the last few decades. The interactive effects of LULC and interannual/long-term climate changes have resulted in …

Erosion And Sediment Controls On Reclaimed Mine Refuse Impoundments, Sara Dalen

Graduate Theses, Dissertations, and Problem Reports

To ensure the sustainable use of one of West Virginia’s precious resources, conscientious long-term management of residual waste is of immense importance. This thesis examined two main topics: i) the dewatering process of acid mine drainage (AMD) sludge, and ii) an investigation of soil loss predictions of a reclaimed coal refuse impoundment.

The first aspect of this thesis addresses clarification and remediation of AMD prior to disposal is a prominent component to preventing runoff and seepage of environmentally detrimental byproducts. To examine the effects of flocculation and dewatering more closely by means of geotextiles, an AMD sludge was analyzed. The …

Recovery Of Phosphorus From Florida Phosphatic Waste Clay, Amir Eskanlou

Graduate Theses, Dissertations, and Problem Reports

This MS thesis examines the recovery of phosphorus (P) from Florida waste clay (WC). A comprehensive literature review revealed that: (i)-The most important values being lost to WC are P and rare earth elements (REEs). For the recovery of these values from WC, two crucial attempts are the removal of extremely fine-sized clays, followed by the recovery of phosphate content, which can pave the path for the recovery of REEs; (ii)-Any scientific/ technological solution should, at the same time, be economically and environmentally attractive to the industry. As such, moving from mostly chemical separation processes to the primarily physical/ physicochemical …

Selective Precipitation And Recovery Of Rare Earth Elements From Acid Mine Drainage, Robyn Lauren Christoferson

Graduate Theses, Dissertations, and Problem Reports

The ability to secure rare earth elements (REEs) in the United States is of concern due to the rapid growth in demand and the monopolistic supply chain. The demand for REEs has skyrocketed in recent years due to the development of many green technologies. The United States and many other countries are currently reliant on China for REEs, who currently control greater than 90% of the global supply. Due to the severe impact of previous Chinese export quotas, it is important to lessen the reliance on importation and explore methods of recycling REEs from secondary sources. A secondary REE source …

Innovative Fe(Iii)-Dosed Anaerobic Biological Treatment System: From Fe And S Biogeochemical Reactions To Engineering Process, Musfique Ahmed

Graduate Theses, Dissertations, and Problem Reports

In moving toward more sustainable wastewater management, anaerobic treatment is gaining increasing popularity due to its simplicity, low energy requirement, low sludge production and less emission of greenhouse gases compared to typical aerobic wastewater treatment systems. Electron acceptors such as nitrate, sulfate, and CO₂ have been used in various anaerobic processes for removal of organic matters from wastewater under anoxic or anaerobic environments. In energy producing regions, ferric iron, Fe(III), is a predominant element in iron containing wastes such as acid mine drainage (AMD) and coal ash, which can potentially be used as a source of iron in novel …

Minimizing Trihalomethane Formation Through Source Water Monitoring And Optimizing Treatment Practices, Nashid Mirza

Graduate Theses, Dissertations, and Problem Reports

Trihalomethanes, a family of halogenated disinfection byproducts, are prevalent in finished drinking water. According to USEPA’s Disinfectants and Disinfection Byproducts Rules (DBPRs), the regulatory standard of the total trihalomethanes (TTHMs) is 80 μg/L, and water treatment plant operators are required to closely monitor TTHMs in the finished water on a quarterly basis. Although total organic carbon (TOC) has been identified as a precursor for the TTHMs, knowledge gaps still exist in how specific water and treatment related factors (e.g., intake water quality, seasonal variation, contact time, and treatment train) affect TTHMs formation. Focusing on water treatment plants in southwestern Pennsylvania, …

Evaluating Design Criteria For High Hazard Dams In A Changing Climate, Aaron Read Sutton

Graduate Theses, Dissertations, and Problem Reports

With changes in climate, there is the potential for future flooding events to vary in frequency and magnitude. These changes may stress the 432 high hazard dames in West Virginia. The 100-year flowrate is an important design criterion for emergency spillways of high hazard dams. Emergency spillways are designed to be reached only by 100-year flow and above. This work quantified how changes in the 100-year flowrate may affect emergency spillway activation. Peakflow data from the Central Appalachian Ecoregion in WV, taken from 24 USGS gages, were used to analyze changes in the 100-year flowrate.

Flow frequency analysis revealed that …

Remotely Sensed Hydroclimatic Variability For Water Security In An Agricultural Basin, Ashish Kondal

Graduate Theses, Dissertations, and Problem Reports

The Indus Basin has supported agricultural practices since the Harappan civilization (3000-1500 BC). Abundance of water has always supported agriculture in the region and built the Indus Basin as one of the major agriculture based economies of the world. The obvious dependency of agriculture on weather and the vagaries of climate emphasizes the necessity of a comprehensive hydro-climate study of the region. The role of climate change in the Indus Basin has always been a contentious issue. Groundwater depletion is evident in the region, and its steady and alarmingly declining rate has brought concerns for the future of agriculture in …

Geotechnical Laboratory Testing For 2-D Fem Analysis Of Geomorphic And Planar Sloped Caps And Covers For Sustainable Mine Refuse Deposition, Sun Hee Park

Graduate Theses, Dissertations, and Problem Reports

This thesis reports the findings of the application of Geomorphic Landform Design principles to the Royal Scot abandoned coarse coal refuse (CCR) pile located in Greenbrier County, West Virginia. The Royal Scot facility has many environmental concerns including severe erosion, acid-mine/rock drainage, and sediment transport. A cap and cover system is proposed to be implemented which incorporates the GLD principles. A two-layer cap and cover system will be used to reclaim the site and return it back to a stable state and reduce the production of acid-mine drainage. The system is composed of a hydraulic barrier composed of compacted CCR, …

Feasibility Of Hydrogen Peroxide Production From Wastewater Treatment Using Bioelectrochemical Systems, Chenjie Wu

Graduate Theses, Dissertations, and Problem Reports

Recent developments of bioelectrochemical systems (BESs) have shown promising advancements in applying these innovative technologies for municipal wastewater treatment. These systems promise several distinct energy and environmental benefits over the existing activated sludge processes including electricity conservation and production, less biological sludge production, significant reduction in greenhouse gas emission, and potential useful chemical production from wastewater treatment. The goal of this research is to evaluate the feasibility of using a two-chambered BES to generate an environmental friendly oxidizer, hydrogen peroxide, from wastewater treatment. Five research objectives were proposed to achieve the research goal by filling several identified knowledge gaps: 1) …