Engineering Commons^™

Adaptive Laboratory Evolution Of Scenedesmus Obliquus For Increased Carbohydrate Content And Biomass Productivity, Nahel Ali

Master's Theses

The economics of microalgal bioproduct commercialization would benefit from increased accumulation of energy storage compounds, such as carbohydrates and lipids, and increased biomass productivity. This thesis explores two adaptive laboratory evolution strategies for improving Scenedesmus obliquus: single UV-mutagenesis and low light cultivation at a high dilution rate to produce cultigens with greater carbohydrate content and iterative UV-mutagenesis and selection under outdoor conditions in a raceway pond at a high dilution rate to increase biomass productivity.

Two cultigens were generated with the goal of increased carbohydrate content: K5 and K7. Both were mutagenized for 5 seconds and cultivated in 50-mL …

Dairy Manure Flushwater Treatment By Algae Raceway Ponds And Aerated Biofilm Reactors, Darin Son

Master's Theses

Nitrogen removal technologies can help dairy industries meet their nutrient management needs. This thesis investigated two separate treatment technologies for total ammonia nitrogen (TAN) removal: algae raceway ponds and aerated biofilm reactors. Six 1000- liter algae raceway ponds and four 1000-liter tote tanks, each equipped with 10 sheets of nonwoven geotextile (i.e., thermally bonded or needle-punched) biofilm substrate, were used to treat the effluent from a flush dairy in central coastal California (TAN = 251 mg/L, cBOD5 = 204 mg/L). For the algae raceway ponds (TAN loading rate = 7 - 35 g/m³-day among 7-, 10- and 14-day hydraulic residence …

Nutrient Removal In Microalgae Raceway Ponds And Nitrification Modeling, Esmeralda Diego

Master's Theses

This thesis explores the treatment of municipal wastewater using pilot-scale raceway ponds and looks specifically at the capability of the raceways in removing BOD and nitrogen. Nine 33 square-meter algal raceway ponds were used to conduct research at the San Luis Obispo Water Resources Recovery Facility. Main objectives of this study were to increase the removal of total ammonia nitrogen (NH₃-N plus NH₄⁺-N) from municipal wastewater through increased assimilation and nitrification. Raceway ponds with CO₂ addition were operated in series with an intermediate settling step and a total hydraulic retention time (HRT) of 4 …