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- Molecules (2)
- Photons (2)
- Point spread functions (2)
- Super resolution microscopy (2)
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- Computational imaging (1)
- Data modeling (1)
- Imaging systems (1)
- Localization microscopy (1)
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- Modulation (1)
- Nanodomains (1)
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- Rotational diffusion (1)
- Sphingomyelinase (1)
- Supported lipid bilayer (1)
Articles 1 - 4 of 4
Full-Text Articles in Engineering
Single‐Molecule 3d Orientation Imaging Reveals Nanoscale Compositional Heterogeneity In Lipid Membranes, Jin Lu, Hesam Mazidi, Tianben Ding, Oumeng Zhang, Matthew D. Lew
Single‐Molecule 3d Orientation Imaging Reveals Nanoscale Compositional Heterogeneity In Lipid Membranes, Jin Lu, Hesam Mazidi, Tianben Ding, Oumeng Zhang, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
In soft matter, thermal energy causes molecules to continuously translate and rotate, even in crowded environments, thereby impacting the spatial organization and function of most molecular assemblies, such as lipid membranes. Directly measuring the orientation and spatial organization of large collections (>3000 molecules μm−2) of single molecules with nanoscale resolution remains elusive. In this paper, we utilize SMOLM, single‐molecule orientation localization microscopy, to directly measure the orientation spectra (3D orientation plus “wobble”) of lipophilic probes transiently bound to lipid membranes, revealing that Nile red's (NR) orientation spectra are extremely sensitive to membrane chemical composition. SMOLM images resolve …
Power Delivery Network As A Physically Unclonable Function, Patrick Naughton, Robert Esswein
Power Delivery Network As A Physically Unclonable Function, Patrick Naughton, Robert Esswein
Electrical and Systems Engineering Capstone Design Projects
Abstract— Physically unclonable functions leverage process variation in the manufacture of silicon chips and circuit boards to map inputs to outputs in an irreversible and unpredictable but consistent manner. They have many applications as security primitives: they can serve as truly random number generators, create secret keys, and fingerprint specific chips. These primitive functions can then be used to secure confidential information and regulate access to private resources. Current approaches tend to utilize variation in the production of silicon dies as the source of variability in their function’s output. We present a PUF that leverages variation in the entire circuit …
Measuring Localization Confidence For Quantifying Accuracy And Heterogeneity In Single-Molecule Super-Resolution Microscopy, Hesam Mazidi, Tianben Ding, Arye Nehorai, Matthew D. Lew
Measuring Localization Confidence For Quantifying Accuracy And Heterogeneity In Single-Molecule Super-Resolution Microscopy, Hesam Mazidi, Tianben Ding, Arye Nehorai, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
We present a computational method, termed Wasserstein-induced flux (WIF), to robustly quantify the accuracy of individual localizations within a single-molecule localization microscopy (SMLM) dataset without ground- truth knowledge of the sample. WIF relies on the observation that accurate localizations are stable with respect to an arbitrary computational perturbation. Inspired by optimal transport theory, we measure the stability of individual localizations and develop an efficient optimization algorithm to compute WIF. We demonstrate the advantage of WIF in accurately quantifying imaging artifacts in high-density reconstruction of a tubulin network. WIF represents an advance in quantifying systematic errors with unknown and complex distributions, …
A Computationally-Efficient Bound For The Variance Of Measuring The Orientation Of Single Molecules, Tingting Wu, Tianben Ding, Hesam Mazidi, Oumeng Zhang, Matthew D. Lew
A Computationally-Efficient Bound For The Variance Of Measuring The Orientation Of Single Molecules, Tingting Wu, Tianben Ding, Hesam Mazidi, Oumeng Zhang, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
Modulating the polarization of excitation light, resolving the polarization of emitted fluorescence, and point spread function (PSF) engineering have been widely leveraged for measuring the orientation of single molecules. Typically, the performance of these techniques is optimized and quantified using the Cramér-Rao bound (CRB), which describes the best possible measurement variance of an unbiased estimator. However, CRB is a local measure and requires exhaustive sampling across the measurement space to fully characterize measurement precision. We develop a global variance upper bound (VUB) for fast quantification and comparison of orientation measurement techniques. Our VUB tightly bounds the diagonal elements of the …