Engineering Commons^™

Confidence Measure For Dna Base Calling Using A Fuzzy System, Rency Susan Varghese

Electronic Theses and Dissertations

Base calling is the central part of any large-scale genomic sequencing effort. Current sequencing technology produces error rates less than 3.5%. This corresponds to at least 35 errors in a 1000 base read. As the base calling algorithm's error rates drop, the smaller base call errors could be difficult to locate. Hence, assembling algorithms and human operators use a confidence value measure to determine how well the base calling algorithm has performed for each base call. This will clearly make it easier to uncover potential errors and correct them, thus increasing the throughput of genetic sequencing. The model developed here …