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Detection Of Prostate Specific Antigen In Serum At The Femto-Gram Per Milliliter Level Using The Intrinsic Amplification Of A Field-Effect Enzymatic Immuno-Sensing System, Jiapeng Wang, Siu-Tung Yau
Detection Of Prostate Specific Antigen In Serum At The Femto-Gram Per Milliliter Level Using The Intrinsic Amplification Of A Field-Effect Enzymatic Immuno-Sensing System, Jiapeng Wang, Siu-Tung Yau
Electrical and Computer Engineering Faculty Publications
Ultrasensitive detection of prostate specific antigen (PSA) has been achieved by applying the field-effectenzymatic detection (FEED) technique to the sandwich immuno-sensing technique. The voltage-controlled intrinsic amplification provided by FEED enabled the detection of PSA contained in serumon the femto-gram/mL level. Two electrochemical approaches used to obtain the amperometric detec-tion signal resulted in similar detection limits and sensitivities. The lowest PSA detection limit achievedwas 27 fg mL-1. The high selectivity of the detection system was reflected in the fact that PSA detectionwas successful on the fg mL-1level, where biological substances other than PSA had a 1-million-foldhigher concentration. Electron transfer through the …
Field-Effect Amperometric Immuno-Detection Of Protein Biomarker, Jiapeng Wang, Siu-Tung Yau
Field-Effect Amperometric Immuno-Detection Of Protein Biomarker, Jiapeng Wang, Siu-Tung Yau
Electrical and Computer Engineering Faculty Publications
The field-effect enzymatic detection technique has been applied to the amperometric immunoassay of the cancer biomarker, carcinoma antigen 125 (CA 125). The detection adopted a reagentless approach, in which the analyte, CA 125, was immobilized on the detecting electrode, which was modified using carbon nanotubes, and the detection signal was obtained by measuring the reduction peak current of the enzyme that was used to label the antibody. A gating voltage was applied to the detecting electrode, inducing increase in the signal current and therefore providing amplification of the detection signal. The voltage-controlled signal amplification of the detection …