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Design And Operation Of A Microwave Flow Cytometer For Single Cell Detection And Identification, Jeffrey A. Osterberg

All Dissertations

Microwave dielectric sensing has become a popular technique in biological cell sensing for its potential in online, label-free, and real-time sensing. At microwave frequencies probing signals are sensitive to intracellular properties since they are able to penetrate cell membranes, making microwave flow cytometry a promising technology for label-free biosensing. In this dissertation a microwave flow cytometer is designed and used to measure single biological cells and micro particles. A radio frequency (RF)/microwave interferometer serves as the measurement system for its high sensitivity and tunability and we show that a two-stage interferometer can achieve up to 20 times higher sensitivity than …