Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Engineering
Strategies For Controlling The Spatial Orientation Of Single Molecules Tethered On Dna Origami Templates Physisorbed On Glass Substrates: Intercalation And Stretching, Keitel Cervantes-Salguero, Austin Biaggne, John M. Youngsman, Brett M. Ward, Young C. Kim, Lan Li, John A. Hall, William B. Knowlton, Elton Graugnard, Wan Kuang
Strategies For Controlling The Spatial Orientation Of Single Molecules Tethered On Dna Origami Templates Physisorbed On Glass Substrates: Intercalation And Stretching, Keitel Cervantes-Salguero, Austin Biaggne, John M. Youngsman, Brett M. Ward, Young C. Kim, Lan Li, John A. Hall, William B. Knowlton, Elton Graugnard, Wan Kuang
Electrical and Computer Engineering Faculty Publications and Presentations
Nanoarchitectural control of matter is crucial for next-generation technologies. DNA origami templates are harnessed to accurately position single molecules; however, direct single molecule evidence is lacking regarding how well DNA origami can control the orientation of such molecules in three-dimensional space, as well as the factors affecting control. Here, we present two strategies for controlling the polar (θ) and in-plane azimuthal (ϕ) angular orientations of cyanine Cy5 single molecules tethered on rationally-designed DNA origami templates that are physically adsorbed (physisorbed) on glass substrates. By using dipolar imaging to evaluate Cy5′s orientation and super-resolution microscopy, the absolute …