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Enhanced Cell Viability And Migration Of Primary Bovine Annular Fibrosus Fibroblast-Like Cells Induced By Microsecond Pulsed Electric Field Exposure, Prince M. Atsu, Connor Mowen, Gary L. Thompson Iii
Enhanced Cell Viability And Migration Of Primary Bovine Annular Fibrosus Fibroblast-Like Cells Induced By Microsecond Pulsed Electric Field Exposure, Prince M. Atsu, Connor Mowen, Gary L. Thompson Iii
Henry M. Rowan College of Engineering Faculty Scholarship
This study is the first to report the enhancement of cell migration and proliferation induced by in vitro microsecond pulsed electric field (μsPEF) exposure of primary bovine annulus fibrosus (AF) fibroblast-like cells. AF primary cells isolated from fresh bovine intervertebral disks (IVDs) are exposed to 10 and 100 μsPEFs with different numbers of pulses and applied electric field strengths. The results indicate that 10 μs-duration pulses induce reversible electroporation, while 100 μs pulses induce irreversible electroporation of the cells. Additionally, μsPEF exposure increased AF cell proliferation up to 150% while increasing the average migration speed by 0.08 μm/min over 24 …