Engineering Commons^™

Separation Technique On Proteins Via A Ph-Parametric Pump : A Theoretical And Experimental Study, Ura Pancharoen

Dissertations

The separation of protein mixtures via pH-parametric pumping was investigated both theoretically and experimentally. A simple system consisting of one column packed either with cation or anion exchanger was first considered. A system of parapumps was then developed with more columns which were connected in series and packed alternately with cation and anion exchangers. Various methods of operation of parapumps are discussed. Enrichment and spliting of protein mixtures were examined. In most cases, the separation factor was defined at a steady state condition and was improved by increasing the number of cycles and columns.

Computational methods for predicting both Batch …

An Experimental Study Of Comparison Between Ph-Parametric Pumping And Ph-Cycling Zone Adsorption Process On Protein Separation, Vincent P. Jajalla

Theses

In this thesis, an experimental investigation was conducted to compare the performance of the continuous pH-parametric pumping and the pH-cycling zone adsorption process on protein separation.

The model system studied was hemoglobin-water. on CM-Sepharose CL-6B (cation) exchanger in a single-column system. Various modes of operation were tried on both the pH-parametric pumping and the pH-cycling zone adsorption method. The separation factors for each mode of operation were computed and compared.

It is shown that the continuous pH-parametric pumping, with circulation to insure the pH uniformity inside the column, has the better separation factor than the pH-cycling zone adsorption method.

Correlation And Prediction Of Vapor-Liquid Equilibrium In Electrolytic Solutions, Anil Krishna Rastogi

Dissertations

Two expressions for the excess Gibbs free energy are presented which correlate and predict vapor-liquid equilibrium and the mean molal activity coefficient of an electrolyte in a ternary mixture containing water and either MeOH or EtOH. The proposed equations take into account coulombic forces between ions and the physical interaction forces between ion-solvent and solvent-solvent molecules in a solution.

Model #1 is a combination of an extended Debye and Huckel equation and the local composition of non-random two liquid (NRTL) model. A ternary mixture requires six adjustable binary parameters to predict activity coefficients. These six parameters are evaluated from three …

Separation Of Proteins Via Continuous Ph Parametric Pumping, Steve Wu

Dissertations

This dissertation covers the separation of proteins via recuperative mode pH parametric pumping. Continuous pH parametric pumping separations using the model system haemoglobin and albumin on a CM-Sepharose cation exchanger have been experimentally and theoretically investigated.

The pH parametric pumping system has a feed containing the protein mixture to be separated, introduced alternately to the top and bottom of the column. The top and bottom products are withdrawn from the apparatus during the bottom and top feed, respectively. Both equilibrium and nonequilibrium cases were considered, and theoretical results agreed with the experimental data.

Buffer concentration, flow rate, reservoir displacement, and …

Parametric Pumping With Ph And Ionic Strength : Enzyme Purification, Zikri Mostafa Ahmed

Dissertations

The work on separation and purification of proteins by parametric pumping has been restricted so far to pH parametric pumping where the difference between the iso-electric points of the components to be separated is apparent. The present work establishes the foundations for the continuous separation and purification of proteins (including enzyms) that have similar or very close iso-electric points by using the ionic strength as a driving force for the separation process. This technique has commercial advantages over batch processes.

It was found that the most promising technique for separating alkaline phosphatase consists of the following three major steps: (I) …

Separation Via Multi-Column Ph Parametric Pumping, Wei Tai Yang

Dissertations

This thesis presents the study of multi-column pH parametric pumping, a separation technique which applies the principle of parametric pumping and uses pH as control variable. Studies emphasize the mass transfer and the capability of separation by multi-column pH parametric pumping. Many operation modes of multi-column pH parapump are developed for varied objectives of separation. A Two-pH Levels Parapump is capable of enriching the product stream with solute components; a Three-pH Levels Parapump is capable of splitting components into two product streams. The separation capability of multi-column parapump is greatly superior to that of single-column parapump. As compared to cycling …

Mathematical Modeling Of Jet Bubbling Reactor, Fred Jyh-Woei Cheng

Theses

A jet bubbling reactor for flue gas desulfurization is described by a mathematical model with the concept of two-stages mass transfer with chemical reactions. The Model consists of two coupling differential equations which are solved simultaneously. The result from the theoretical model is agreeable SO₂ removal data obtained from a pilot plant reactor.

The Heterogeneous Catalytic Decomposition Of Isopropyl Alcohol Over 0.5% Platinum On 4-8 Mesh Charcoal Catalyst Support, Thomas Michael Kudenchak

Theses

The heterogeneous catalytic decomposition of isopropyl alcohol vapor over 0.5% platinum on (4-8 mesh) charcoal support catalyst was studied at bench-top in a fixed-bed tubular micro-reactor of about 1 cm ID, six inches long containing one or two grams of catalyst. Reactor feed and exit gas analysis was obtained by gas chromatography.

Experimental runs aimed at determining the Arrhenius parameters and the reaction order (assuming the applicability of a simple power law model) were performed with 1 and 2 grams of catalyst at various residence times ranging from 0.24 to 2.3 seconds and temperatures of 150°, 175°, 200°, 225°, and …

A Determination Of The Energy Storage Capacity Of The Diels-Alder Reaction Between Methylfuran And Maleic Anhydride As Applied To Storing Solar Energy, Brent Gregory Sparks

Masters Theses

“The heat storage capacity of the Diels-Alder reaction between 2-methylfuran and maleic anhydride is calculated using reaction parameters obtained from solution calorimetry. An equilibrium constant of .614 1/mol and a heat of reaction of 14.33 Kcal/mole were obtained from experiments at 45°C. A reaction ΔC_p of -21.8 cal/mole was calculated from heat capacity information at 25°C. From these parameters, a solution initially seven molar in methylfuran and maleic anhydride was found to have a maximum apparent volumetric heat capacity of about 1.85 times that of water. This maximum occurs at about 335 K. Typical active solar energy schemes operate …