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Biohydrogen Production From Cellulose By Clostridium Termitidis And Clostridium Beijerinckii., Maritza Gomez-Flores

Electronic Thesis and Dissertation Repository

The purpose of this study was to determine Clostridium termitidis microbial kinetics on glucose, cellobiose, and cellulose, and to assess its co-culture with Clostridium beijerinckii for hydrogen production. Microbial kinetics parameters of the mesophilic, cellulolytic, and hydrogen producer C. termitidis were determined on glucose and cellobiose using MATLAB for modelling biomass growth and substrate consumption. Hydrogen yields on these substrates were 1.99 and 1.11 mol H₂ mol^-1 hexose equivalent, respectively. C. termitidis microbial kinetics in mono-culture and in co-culture with mesophilic hydrogen producer C. beijerinckii were also investigated under agitated and non-agitated conditions, with hydrogen yields of 1.46 …